U.T. Bornscheuer / R.J. Kazlauskas, 2nd edition, Wiley-VCH (2005)
Due to a major problem with MS Word® in the creation of the camera-ready version of the 2nd edition, the index of the printed book refers to wrong pages!
The correct index can be downloaded here (pdf).
Review of the 2nd edition by Per Berglund in ChemBioChem (pdf)
Preface to the 2nd edition
Over
the decade, hydrolases have become key parts of the growing area of industrial
biotechnology (Schmid et al., 2001; Shoemaker et al., 2003). We hope that
the first edition of Hydrolases in Organic Syntheses contributed to this success.
It was a key reference in our laboratories and an excellent starting point
for new researchers in the field. For this success to continue, we felt that
we had to keep it current with new developments in the area – directed
evolution and protein design, dynamic kinetic resolutions, and the use of
structures to explain selectivity.
Planning the 2nd edition presented a challenge – more than 900 publications
in the area since 1999. We chose to focus on new areas, new insights, and
new frontiers and not to focus on comprehensive lists of substrates. For this
information, databases are more efficient than a book. We've added a new chapter
on protein sources and optimization of biocatalyst performance for organic
synthesis, an expanded chapter about directed evolution and a new chapter
about catalytic promiscuity. In addition, we expanded sections dynamic kinetic
resolution and immobilization and added new classes of hydrolases, such as
haloalkane dehalogenases and organophosphorus hydrolases. In turn, we shortened
some sections including a major shortening of the sections on lipid modification,
which is of limited interest to organic chemists. These changes required a
rearrangement of the book chapters. In addition, many sections contain minor
updates.
We sincerely hope that these changes will extend and enhance the usefulness
of this book.
1st edition (1999)
Preface
to the 1st edition
Each traveller to a city seeks something different.
One wants to see that special painting in the museum, another wants to drink
the local beer, a third wants to meet a soulmate. Each organic chemist also
seeks something different from the field of biocatalysis. One wants high enantioselectivity,
another wants reaction under mild conditions, a third wants to scale up to
an industrial scale. We hope this book can be a guide to organic chemists
exploring the field of biocatalysis. Enzyme-catalyzed reactions, especially
hydrolase-catalyzed reactions, have already solved hundreds of synthetic problems
usually because of their high stereoselectivity.
The organization is aimed at the chemist ? by reaction type and by different
functional groups. This information should help organic chemists identify
the best hydrolase for their synthetic problem. In addition, we suggest how
to choose an appropriate solvent, acyl donor, immobilization technique and
other practical details. We hope that learning how others solved synthetic
problems will generate ideas that solve the next generation of problems.
Although this book has more than 1 700 references, we might have missed important
hydrolase-catalyzed reactions. The choices on what to include usually reflect
our own research interests, but were sometimes arbitrary or even inadvertent.
Updates will be posted on our website:
http://pasteur.chem.mcgill.ca/hydrolases.html
For further information please contact:
http://www.wiley-vch.de
What others think about
our book:
Fritz Theil, Angew. Chem.: in German
or in English (pdf)
Nick Turner, Org. Proc. Res. Develop. English
(pdf)
News
We organize two conferences in 2012 at our Institute:
Aug. 29-31st 2012:
Protein Engineering for Biocatalysis
Sept. 19-21st 2012:
Biocatalysis in Lipid Modification
Our publication made the cover of Nature Chem. Biol, Nov. 2010:
Author profile:
An author profile about Uwe Bornscheuer has been published in Angew. Chem. Int. Ed.
Flyer available:
English version (pdf)
German version (pdf)
Cluster Partner:
Partner in the 'White Biotechnology' cluster 'Biokatalyse2021' funded by the BMBF.
Contact
Prof. Dr.
Uwe Bornscheuer
Institute of Biochemistry
Dept. of Biotechnology & Enzyme Catalysis
Felix-Hausdorff-Str. 4
D-17487 Greifswald/ Germany
Phone:
+49 (0)3834-86-4367
+49 (0)3834-86-4391 (Secr.)
Fax:
+49 (0)3834-86-794367
E-Mail:
uwe.bornscheuer
