Prof. Dr. Uwe T. Bornscheuer

Books

 

 

 

Book chapters

[30] Kazlauskas, R.J., Bornscheuer, U.T. (2012) Enzyme catalytic promiscuity: explanding the catalytic action of enzymes to new reactions. In: Comprehensive Chirality, Yamamoto, H., Carreira, E. (Eds.), accepted.

[29] Geitner, K., Bornscheuer, U.T. (2012), Large scale Baeyer-Villiger monooxygenase-catalyzed conversion of (R,S)-3-phenylbutan-2-one. In: Practical Methods in Biocatalysis and Biotransformations, Sutton, P., Whittall, J. (Eds.), Wiley-VCH, Weinheim, accepted.

[28] Bornscheuer, U.T., Kazlauskas, R.J. (2012) Enzymatic catalytic promiscuity and the design of new enzyme catalyzed reactions In: Enzymes in Organic Synthesis, May, O., Gröger, H., Drauz, W. (Eds.), Wiley-VCH, Weinheim, accepted.

[27] Höhne, M., Bornscheuer, U.T., (2012) Application of transaminases in organic synthesis. In: Enzymes in Organic Synthesis, May, O., Gröger, H., Drauz, W. (Eds.), Wiley-VCH, Weinheim, accepted.

[26] Bornscheuer, U.T. (2012) Biocatalytic processes. In: Catalysis: From Molecular to Reactor Design, Beller, M., Renken, A., van Santen, R.A. (Eds.), Wiley-VCH, Weinheim, accepted.

[25] Bornscheuer, U.T. (2012) Biocatalysis. In: Catalysis: From Molecular to Reactor Design, Beller, M., Renken, A., van Santen, R.A. (Eds.), Wiley-VCH, Weinheim, accepted.

[24] Bornscheuer, U.T. (2010), Lipases, applications in synthesis of chiral compounds and in organic solvents, Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation and Cell Technology (Flickinger, M.C. Ed.)., John Wiley & Sons, Hoboken, link.

[23] Rehdorf, J., Bornscheuer, U.T. (2010), Monooxygenases, Baeyer-Villiger oxidations in organic synthesis, Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation and Cell Technology (Flickinger, M.C. Ed.)., John Wiley & Sons, Hoboken, link.

[22] Bornscheuer, U.T. (2010), Evolving enzymes for biocatalysis. In: Microbiology of Hydrocarbons, Oils, Lipids and Derived Compounds, Timmis, K. (Ed.), Springer, Berlin, 2930-2938.

[21] Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2010), Directed evolution of industrial biocatalysts. In: Industrial Biotechnology, Soetaert, W., Vandamme, E. (Eds.), Wiley-VCH, Weinheim, pp. 173-205.

[20] Kirschner, A., Bornscheuer, U.T. (2009), Baeyer-Villiger monooxygenases in organic synthesis. In: Green Chemistry, Vol. 3: Biocatalysis, Anastas, P.T. (Ed.), Wiley-VCH, Weinheim, pp. 115-149.

[19] Böttcher, D., Schmidt, M., Bornscheuer, U.T. (2009), Alteration of substrate specificity and stereoselectivity of lipases and esterases. In: Protein Engineering, Bornscheuer, U.T., Lutz, S. (Eds.), Wiley-VCH, Weinheim, pp. 753-775.

[18] Kirschner, A., Bornscheuer, U.T. (2009), Enantioselective kinetic resolution of racemic 3-phenylbutan-2-one using a Baeyer-Villiger monooxygenase. In: Practical Methods in Biocatalysis and Biotransformations, Roberts, S.M., Whittall, J. (Eds.), Wiley-VCH, Weinheim, pp. 337-340.

[17] Hidalgo, A., Bornscheuer, U.T. (2006), Directed evolution of lipases and esterases for organic synthesis. In: Biocatalysis in the Pharmaceutical and Biotechnological Industries (Ed. R. Patel), Marcel Dekker, New York, pp. 159-179.

[16] Bornscheuer, U.T., Henke, E., Pleiss, J. (2005), Enzymatic Methods. In: Quaternary Stereocenters (Ed. Christoffers, J., Baro, A.), Wiley-VCH, Weinheim, pp. 315-327.

[15] Bornscheuer, U.T. (2005), Trends and Challenges in Enzyme Technology, Adv. Biochem. Eng. / Biotechnol., 100, 181-203.

[14] Bornscheuer, U.T. (2003), Assay systems for screening or selection of biocatalysts. In: Enzyme functionality: Design, engineering and screening (Svendsen, A, ed.) Marcel Dekker, New York, pp. 475-492.

[13] Bornscheuer, U.T., Adamczak, M., Soumanou, M.M. (2002) Lipase-catalyzed synthesis of modified lipids. In: Lipids as constituents of functional foods (Gunstone, FD, ed.) PJ Barnes & Associates, Bridgwater, pp. 149-182.

[12] Bornscheuer, U.T. (2002), Evolutionary Generation of Enzymes with Novel Substrate Specificities, in: Directed Evolution of Proteins; (Brakmann, S., Johnsson, K.; Ed.), Weinheim: Wiley-VCH, pp. 329-341.

[11] Yan, Y., Bornscheuer,U.T., Schmid, R.D. (2001), Application of crystallization technique for the lipase-catalyzed solid phase synthesisof sugar fatty acid monoesters; (Wildlak, N., Hartel, R., Narine, S.; Ed.),Champaign: AOCS Press, Vol. 15, 168-175.

[10] Schwaneberg, U., Bornscheuer, U.T. (2000), Fatty acid hydroxylations using P450 monooxygenases, in: Enzymes in Lipid Modification (Bornscheuer, U.T., Ed), Weinheim: Wiley-VCH, pp.394-414.

[9] Bornscheuer,U.T. (2000), Strategies for Improving the Lipase-Catalysed Preparation of Chiral Compounds, in: Methods and Tools in Biosciences and Medicine,Methods in Non-aqueous Enzymology (Gupta, M.N.; Ed.), Basel: Birkhäuser,pp. 90-109.

[8] Bornscheuer,U.T. (2000), Industrial Biotransformations, in: Biotechnology-Series(Rehm,H.J., Reed, G., Pühler, A. Stadler, P.J.W.; Eds.), Vol.8b, Weinheim: Wiley-VCH, pp. 277-294.

[7] Bornscheuer, U.T. (1999), ~250 keywords, in: Dictionary of Biotechnology and Gentechnology (in german language) (Deckwer, W.D., Pühler, A., Schmid, R.D.; Eds.). Stuttgart: Thieme, ISBN: 3137364027

[6] Bornscheuer, U.T., Pleiss, J., Schmidt-Dannert, C., Schmid, R.D. (1999), Lipases from Rhizopus oryzae: genetics, structure and applications, in.: Protein Engineeringin Industrial Biotechnology (Alberghina, L.; Eds.), 115-134. London: Harwood.

[5] Bornscheuer, U.T. (1999), Extending the applicability of lipases and esterases for organic synthesis, in.: Bioorganic Chemistry - Highlights and New Aspects (Diederichsen, U., Lindhorst, T.K., Wessjohann, L. , Westermann, B.; Eds.) (1999), Weinheim: Wiley-VCH, ISBN 3-527-29665-4, pp. 127-131.

[4] Bornscheuer, U.T. (1999), Recent advances in the lipase-catalyzed biotransformation of fats and oils, in.: Recent Research Developments in Oil Chemistry,Vol. 3,93-106. Trivandrum: Transworld Research Network.

[3] Bornscheuer, U., Padmanabhan, P., Scheper, T. (1999), Emulsion immobilized enzymes, in: Microspheres, microcapsules & liposomes (Arshady, R.; Ed.) 541-558. London: Citus Books.

[2] Kazlauskas, R.J., Bornscheuer, U.T. (1998), Biotransformations with Lipases, in.: Biotechnology-Series (Rehm, H.J., Reed, G., Pühler, A., Stadler, P.J.W., Kelly, D.R.; Eds.), Vol.8a, 37-191. Weinheim: Wiley-VCH.

[1] Soumanou, M.M., Schmid, U., Bornscheuer, U.T., Schmid, R.D. (1997), Lipasekatalysierte Synthesevon strukturierten Triglyceriden, in: Biokonversion nachwachsender Rohstoffe ,Vol.10, 184-190. Münster: Landwirtschaftsverlag.

Book reviews
[7] Dynamic Stereochemistry of Chiral Compounds. Principles and Applications, by C. Wolf, RSC Publishing, Cambridge, reviewed in Angew. Chem. Int. Ed. (2008), 47, 5282; Angew. Chem. (2008), 120, 5362.

[6] Biocatalysis, by A. Bommarius, B.R. Riebel, Wiley-VCH, Weinheim, ISBN 3-527-30344-8, reviewed in Angew. Chem. Int. Ed. Engl. (2004), 43, 6584-6585; Angew. Chem. (2004), 116, 6746-6747.

[5] Taschenatlas der Biotechnologie und Gentechnik, by R.D. Schmid, Wiley-VCH, Weinheim, ISBN 3-527-30865-2, reviewed in Angew. Chem. (2002), 114, 3435.

[4] Enzymes: Biochemistry, Biotechnology, Clinical Chemistry. By T. Palmer. Horwood, Chichester, UK, ISBN 1-898563.78.0, reviewed in Angew. Chem. (2001), 113, 3358; .Angew. Chem. Int. Ed. Engl. (2001), 40, 3258-3259.

[3] Aqueous Two-Phase Systems. Methods and Protocols. Edited by R. Hatti-Kaul, Humana Press, Totawa, ISBN 0-896-03541-7, reviewed in Angew. Chem. (2000), 112, 4556-4557; .Angew. Chem. Int. Ed. Engl. (2000), 39, 4384-4385.

[2] Synthesis in Lipid Chemistry. Edited by J.H.P. Tyman, Royal Society of Chemistry, Cambridge, UK, ISBN 0-85404-716-6, reviewed in Fett/Lipid (1998), 100, 27.

[1] Lipoxygenase and Lipoxygenase Pathway Enzymes. Edited by G. J. Piazza, AOCS Press, Champaign, IL, USA, ISBN 0-935315-74-8, reviewed in Fett/Lipid (1997), 99, 152.

Others

[13] Bornscheuer, U.T. (2011) Can synthetic biology/metabolic engineering contribute to the microbial production of triglycerides and oleochemicals?, Eur. J. Lipid Sci. Technol., 113, 1075-1076, link.

[12] Khademhosseini, A., Bornscheuer, U.T. (2011) Engineering of pathways, cells and tissues, Curr. Opin. Biotechnol., 22, 601-603, link.

[11] Weckhuysen, B.M., Oro, L.A., Bornscheuer, U.T. (2011) Solving the material and energy challenges of the future, ChemCatChem, 3, 619-621, link.

[10] Antranikian, G., Bornscheuer, U.T., Liese, A. (2010) Highlights in Biocatalysis, ChemCatChem, 2, 879-880, link.

[9] Daum, G., Bornscheuer, U.T., Amoneit, F. (2010), Meeting report of the 7th Euro Fed Lipid Congress, Graz, Austria, 2009. Eur. J. Lipid Sci. Technol., 112, 275-287, link.

[8] Bornscheuer, U.T. (2010), Looking back into the future, Eur. J. Lipid Sci. Technol., 112, 153-154, link.

[7] Bornscheuer, U.T. (2009) Combined success for efficient catalysis, ChemCatChem, 1, 5, link.

[6] Bornscheuer, U.T. (2009) Author profile, Angew. Chem. Int. Ed. 48, 5236, link; Angew. Chem.121, 5338, link.

[5] Kolisis, F., Bornscheuer, U.T., Amoneit, F. (2009), Report of the 6th Euro Fed Lipid Congress, Athens, Greece, 2008. Eur. J. Lipid Sci. Technol., 111, 303-309.

[4] Bornscheuer, U.T. (2009), Meeting report: Protein Design and Evolution for Biocatalysis. Biotechnol. J., 4, 444-446, link.

[3] Bornscheuer, U.T. (2008), Taking over a safe sailing boat heading to new discoveries, Eur. J. Lipid Sci. Technol., 110, 1, link.

[2] Bornscheuer, U.T. (2007), Editorial: Protein design and evolution for biocatalysis (special issue), Biotech. J., 2, 155, link.

[1] The Biocatalysis and Biodegradation Database, reviewed in Angew. Chem. (2001), 113, 3817 (pdf); Angew. Chem. Int. Ed. Engl. (2000), 40, 3703 (pdf).

Patents (open to the public)

[32] Bornscheuer, U.T, Vorhaben, T., Böttcher, D., Jasinski, D., Menyes, U., Häckel, M., Schröder, K. (2011) Immobilisierung von Enzym unter Verwendung von Plasmen, Patent PCT WO 2011131594.

[31] Bornscheuer, U.T. Gerstenbruch, S., Mußmann, N., Maurer, K.-H., O'Connell, T. (2010) Mutanten einer Sorbitoloxidase mit verbesserter spezifischer Aktivität auf Glycerin, German Patent Application DE10 2010 028008.

[30] Bornscheuer, U., Hesseler, M. (2011), Method for the conversion of 3-halogen-1,2-propanediol and its mono- and diester derivatives to 1,2,3-propanetriol, Patent Applications DE 10 2009 042 760.0; WO 2011036072.

[29] Robins, K., Höhne, M., Bornscheuer, U.T. (2011), Screening, purification and characterization of microbial (R)-specific ω- transaminases and use for asymmetric synthesis of (R)-amines, Patent PCT WO 2011026556.

[28] Bornscheuer, U., Böttcher, D., Brüsehaber, E., Doderer, K. (2010), Recombinant production of pig liver esterase in E. coli using GroEL/GroES chaperone system, US Patent application.

[27] Bornscheuer, U.T., Hummel, A., Böttcher, D., Brüsehaber, E., Doderer, K., Trauthwein, H. (2008), New variants of pig liver esterase gamma for use in stereospecific hydrolysis. Intern. Patent PCT WO 2008116745; German Patent DE 102007014742.

[26] Robins, K., Bornscheuer, U., Höhne, M. (2008) Process for preparation of optically active n-protected 3 -aminopyrrolidine or optically active n-protected 3-aminopiperidine and the corresponding ketones by optical resolution of the racemic amine mixtures employing a bacterial omega-transaminase. Intern. Patent PCT WO 2008028654.

[25] Bornscheuer, U.T., Kirschner, A. (2008), Kinetic resolution of β-hydroxyketones by a cloned Baeyer-Villiger monooxygenase from Pseudomonas flourescens. Granted patent: DE 102006039189 B4 20100729; Intern. Patent PCT WO 2008022.

[24] Liebeton, K., Eck,. J., Bornscheuer, U.T., Böttcher, D., Langer, P., Bellur, E. (2007), A novel group of esterases for the enantioselective production of fine and specialty chemicals, Intern. Patent PCT WO 2007128496.

[23] Robins, K., Bornscheuer, U., Höhne, M. (2007), Process for the preparation of optically active chiral amines. European Patent EP 1818411.

[22] Jackisch, B.O., Simmler-Hübenthal, H., Zschau, W., Bornscheuer, U.T., Durban, M., Riemer, C. (2007), Use of a thermostable phospholipase in the degumming of an oil or fat, and a method for obtaining a thermostable phospholipase, European Patent EP 1788080.

[21] Bornscheuer, U.T., Schmidt, M., Kähler, M., Rieks, A. (2006), New esterases and their use for processes for kinetic resolution of butinolesters, Intern. Patent PCT WO 07/042444.

[20] Bornscheuer, U.T., Grüning, B., Hills, G., Möbius, P., Thum, O., Weitemeyer, C. (2005), Process for the enzymatic synthesis of ethers. German Patent DE102005043669, US Patent 2007059789.

[19] Bornscheuer, U.T., Weiner, D.P., Hitchman, T., Lyon, J., Wongsakul, S. (2005), Hydrolases, nucleic acids encoding them and mehods for making and using them. European Patent EP 1601332; International Patent PCT WO 05/032496.

[18] Bornscheuer, U.T., Musidlowska-Persson, A., Trauthwein, H. (2004), Artificial esterases. German Patent DE 10258327.

[17] Bornscheuer, U.T., Konarzycka, M., Hills, G. (2003). Assay for synthesis activity of hydrolases in nonaqueous media using enzymic transesterification reaction releasing a carbonyl compd. European Patent EP 1354960.

[16] Bornscheuer, U.T., Baumann, M. (2002), Detection method for identifying hydrolases. German Patent DE 101 24 799; European Patent EP 1395673; International Patent PCT WO 02/94949.

[15] Short, J., Mathur, E., Baumann, M., Bornscheuer, U. (2002), Hydrolase enzymes and their use in kinetic resolution. International Patent WO 02/057411.

[14] Tscherry, B., Bornscheuer, U., Musidlowska, A., Werlen, J. Zimmermann, T. (2002), Manufacture of L-carnitine from betaine esters with microorganisms bearing a betaine ester hydrolase. European Patent EP 1370663; International Patent PCT WO 02/061094; TW 316547 B 20091101.

[13] Riermeier, T., Bornscheuer, U., Altenbuchner, J., Hildebrandt, P. (2002), An alcohol dehydrogenase from Pseudomonas fluorescens for use in the manufacture of alcohols from ketones. German Patent DE 10112401; European Patent EP 1241263.

[12] Bornscheuer, U., Musidlowska, A., Schmidt-Dannert, C., Lange, S. (2002), Rekombinante Schweineleberesterase, deren Verwendung sowie ein Verfahren zu deren Herstellung. German Patent DE 10061864; European Patent EP 1343902; International Patent PCT WO 02/048322.

[11] Wessjohann L.A., Scheid G., Bornscheuer U., Henke E., Kuit W., Orru R. (2002), Epothilone synthesis components III and IV: Asymmetrically substituted acyloins and acyloin derivatives, method for the production thereof and method for the production of Epothilone B, D and Epothilone derivatives. German Patent DE 10134172 ; European Patent EP 1358144; US Patent US 6,867,333; International Patent PCT WO 02/32844.

[10] Bornscheuer, U.T., Meyer, H.H., Altenbuchner, J., (2002), Alteration of substrate specificity of enzymes, German Patent DE 19743683; European Patent EP 0909821; US Patent US 6,365,398.

[9] Gatfield, I., Hilmer, J.M., Bornscheuer, U., Schmid, R.D., Vorlova, S., (2002), Method for preparing D- and L-menthol. German Patent DE 10100913; European Patent EP 1223223; US Patent US 6,706,500.

[8] Brocca, S., Bornscheuer, U., Pleiss, J., Schmid, R.D., Schmid, U., Schmitt, J. Modified lypolytic enzymes and their use. European Patent EP 1130100.

[7] Bornscheuer, U., Henke, E., Yang, H. (2002), Verfahren zur Herstellung von stereoisomeren Carbonsäureestern, German Patent DE 19908074; European Patent EP 1031629; US Patent US 6,365,398.

[6] Yang,H., Henke, E., Bornscheuer, U.T. (2000), Verfahren zur Racematspaltung von Arylalkylcarbonsäureestern. German Patent DE 19844876; European Patent EP 0997534; US Patent US 6,201,147.

[5] Bornscheuer, U.T, Schmid, R.D., Scheib, H., Otto, R.T., Syldatk, C. (2000), Method for the production of uronic acid esters in a lipase-catalyzed process. German Patent DE 19839219.

[4] Bornscheuer, U., Schmid, R.D., Syldatk, C., Youchun, Y., Otto, R. (2000), Verfahren zur selektiven Veresterung von Polyolen. German Patent DE 19924221.

[3] Otto, R., Syldatk, C., Cao, L., Bornscheuer, U., Schmid, R.D. (1999), Verfahren zur selektiven Veresterung von Polyolen. German Patent DE 19753789; European Patent EP 1175500.

[2] Bornscheuer, U.T., Soumanou, M.M., Schmid, R.D., Schmid, U. (1998), Preparation of symmetrical triglycerides ABA, European Patent EP 0882797.

[1] Minning, S., Bornscheuer, U., Schmid, R.D. (1998), Azo dye for detection of percarboxylic acids, German Patent DE 19651797.

 

 

 

Publication and Patent List

(for a listing as pdf-file click here)

• Books (4)
• Book chapters (30)
• Book reviews (7)
• Others (13)
• Patents (32)
• Peer-reviewed papers (263)
  • accepted
  • 2012
  • 2011
  • 2010
  • 2009
  • 2008
  • 2007
  • 2006
  • 2005
  • 2004
  • 2003
  • 2002
  • 2001
  • 2000
  • 1999
  • 1998
  • 1997
  • 1996
  • 1995
  • 1994
  • 1993
  • 1992
  • 1991

 

accepted

[263] Smith, M.E., Banerjee, S., Shi, Y., Schmidt, M., Bornscheuer, U.T. Masterson, D.S. (2012), Investigation of the cosolvent effect on six isoenzymes of PLE in the enantioselective hydrolysis of selected a,a-disubstituted malonate esters, ChemCatChem, accepted.

 

2012

[262] Schließmann, S., Schmidt, M., Torres, L.L., Gámez, J.A., Silva-Martín, N., Hermoso, J.A., Berenguer, J., Bornscheuer, U.T., Hidalgo, A. (2012) Promiscuous lactamase activity in an esterase originates from electronic, steric and mechanistic determinants, Org. Biomol. Chem., link.

[261] Brundiek, H., Sass, S., Evitt, A., Kourist, R., Bornscheuer, U.T. (2012), The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans fatty acid selectivity, Appl. Microb. Biotechnol., link.

[260] Gerstenbruch, S., Wulf, H., Mußmann, N., O'Connell, T., Maurer, K.-H., Bornscheuer, UT. (2012), Directed evolution of an alditol oxidase from Streptomyces coelicolor for enhanced oxidative activity towards glycerol, Appl. Microb. Biotechnol., link.

[259] Kadow, M., Loschinski, K., Saß, S., Schmidt, M., Bornscheuer, U.T. (2012), Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli and biochemical characterization, Appl. Microb. Biotechnol., link.

[258] Pavlidis, I.V., Vorhaben, T., Tsoufis, T., Rudolf, P., Bornscheuer, U.T., Gournis, D., Stamatis, H. (2012) Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials, Biores. Technol., link.

[257] Brundiek, H.B., Evitt, A.S., Kourist, R., Bornscheuer, U.T. (2012), Creation of a lipase highly selective for trans fatty acid by protein engineering, Angew. Chem. Int. Ed., 51, 412-414, link; Erzeugung einer für trans-Fettsäuren hochselektiven Lipase durch Protein-Engineering, Angew. Chem., 124, 425-428, link.

[256] Leipold, F., Wardenga, R., Bornscheuer, U.T. (2012), Cloning, expression and characterisation of an eukaryotic cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011, Appl. Microb. Biotechnol., link.

[255] Rehdorf, J., Behrens, G.A., Nguyen, G.-S., Kourist, R., Bornscheuer, U.T. (2012), Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols, Appl. Microb. Biotechnol., 93, 1119-1126, link.

[254] Wulf, H., Perzborn, M., Sievers, G., Scholz, F., Bornscheuer, U.T. (2012), Kinetic resolution of glyceraldehyde using an aldehyde dehydrogenase from Deinococcus geothermalis DSM11300 combined with electrochemical cofactor recycling, J. Mol. Catal. B: Enzymatic, 74, 144-150, link.

2011 (total IF: 86.119)

[253] Vorhaben, T., Häckel, M., Bornscheuer, U.T., Menyes, U. (2011) Plasmagestützte Immobilisierung von Enzymen, Nachrichten Chemie, 59, 1147-1149.

[252] Jonczyk, P., Schmidt, A., Bice, I., Lück, M., Gross, E., Hilmer, J.M., Bornscheuer, U., Beutel, S., Scheper, T. (2011), Strikt anaerobe batch-Kultivierung von Eubacterium ramulus in neuartigem Einweg-Beutelreaktorsystem, Chem. Ing. Techn., 83, 2147-2152, link.

[251] Bornscheuer, U.T., Kazlauskas, R.J. (2011), Survey of protein engineering strategies, Curr. Prot. Prot. Sci., 66, 26.7.1-26.7.14, link.

[250] Herter, S., Mikolasch, A., Michalik, D., Hammer, E., Schauer, F., Bornscheuer, U.T., Schmidt M. (2011), C-N coupling of 3-methylcatechol with primary amines using native and recombinant laccases from Trametes versicolor and Pycnoporus cinnabarinus, Tetrahedron, 67, 9311-9321, link.

[249] Behrens, G.A., Hummel, A., Padhi, S.K., Schätzle, S., Bornscheuer, U.T. (2011) Discovery and protein engineering of biocatalysts for organic synthesis, Adv. Synth. Catal., 353, 2191-2215, link.

[248] Schätzle, S., Steffen-Munsberg, F., Thontowi, A., Höhne, M., Robins, K., Bornscheuer, U.T. (2011), Enzymatic asymmetric synthesis of enantiomerically pure aliphatic, aromatic and arylaliphatic amines with (R)- selective amine transaminases, Adv. Synth. Catal., 353, 2439-2445, link.

[247] Fernández-Àlvaro, E., Snajdrova, R., Jochens, H., Davids, T., Böttcher, D., Bornscheuer, U.T. (2011), A combination of in vivo selection and cell sorting for the identification of enantioselective biocatalysts, Angew. Chem. Int. Ed., 50, 8584-8587, link; Eine Kombination aus In-vivo-Selektion und Zellsortierung zur Identifizierung enantioselektiver Biokatalysatoren, Angew. Chem., 123, 8742-8746, link.

[246] Kadow, M., Saß, S., Schmidt, M., Bornscheuer, U.T. (2011), Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007, AMB Express, link.

[245] Kourist, R., Bornscheuer, U.T. (2011), Biocatalytic synthesis of optically active tertiary alcohols, Appl. Microb. Biotechnol., 91, 505-517, link.

[244] Palm, G.J., Fernández-Alvaro, E., Bogdanovic, X., Bartsch, S., Sczodrok, J., Böttcher, D., Singh, R.K., Atomi, H., Bornscheuer, U.T., Hinrichs, W. (2011), The crystal structure of an esterase from the hyperthermophilic microorganism Pyrobaculum calidifontis VA1 supports explanation of its enantioselectivity, Appl. Microbiol. Biotechnol., 91, 1061-1072, link.

[243] Hesseler, M., Bogdanovic, X., Hidalgo, A., Berenguer, J., Palm, G., Hinrichs, W., Bornscheuer, U.T. (2011), Cloning, functional expression, biochemical characterization and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1, Appl. Microb. Biotechnol., 91, 1049-1060, link.

[242] Jochens, H., Hesseler, M., Stiba, K., Padhi, S.K., Kazlauskas R.J., Bornscheuer, U.T. (2011), Protein engineering of α/β-hydrolase fold enzymes, ChemBioChem, 12, 1508-1517, link.

[241] Ni, Y., Li, C.X., Zhang, J., Shen, N.-D., Bornscheuer, U.T., Xu, J.H. (2011), Efficient reduction of ethyl 2-oxo-4-phenylbutyrate at 620 g/L by a bacterial reductase with broad substrate spectrum, Adv. Synth. Catal., 353, 1213-1217, link.

[240] Cassimjee, K.E, Kourist, R., Lindberg, D., Wittrup-Larsen, M., Nguyen, H.T., Widersten, M., Bornscheuer, U.T., Berglund, P. (2011), One-step enzyme extraction and immobilisation for biocatalysis applications, Biotechnol. J., 6, 463-469, link.

[239] Evitt, A., Bornscheuer, U.T. (2011), Lipase CAL-B does not catalyze a promiscuous decarboxylative aldol addition or Knoevenagel reaction, Green Chem., 13, 1141-1142, link.

[238] Biermann, U., Bornscheuer, U.T., Meier, M.A.R., Metzger, J.O., Schäfer, H.J. (2011) New developments for the chemical utilization of oils and fats as renewable raw materials, Angew. Chem. Int. Ed., 50, 3854-3871, link; Neue Entwicklungen zur stofflichen Nutzung von Fetten und Ölen als nachwachsende Rohstoffe, Angew. Chem., 123, 3938-3956, link.

[237] Nguyen, G.S., Thompson, M.L., Grogan, G., Bornscheuer, U.T., Kourist, R. (2011), Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining, J. Mol. Catal. B: Enzymatic, 70, 88-94, link.

[236] Herter, S., Nguyen, G.S., Thompson, M.L., Steffen-Munsberg, F., Schauer, F., Bornscheuer, U.T., Kourist, R. (2011), Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries, Appl. Microbiol. Biotechnol., 90, 929-939, link.

[235] Hasenpusch, D., Bornscheuer, U.T., Langel, W. (2011), Simulation on the structure of pig liver esterase, J. Mol. Model., 17, 1493-1506, link.

[234] Schwenteit, J., Gram, L., Nielsen, K.F., Fridjonsson, O.H., Bornscheuer, U.T., Givskov, M., Gudmundsdottir, B.K. (2011), Quorum sensing in Aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase AsaI on bacterial virulence, Vet. Microbiol., 147, 389-397, link.

2010 (total IF: 114.381)

[233] Bartsch, S., Bornscheuer, U.T. (2010) Mutational analysis of phenylalanine ammonia lyase to improve reaction rates for various substrates, Protein Eng. Des. Sel., 23, 929-933, link.

[232] Höhne, M., Schätzle, S., Jochens, H., Robins, K., Bornscheuer, U.T. (2010) Rational assignment of key motifs for function guides in silico enzyme identification, Nature Chem. Biol., 6, 807-813, link.

[231] Jochens, H., Aerts, D., Bornscheuer, U.T. (2010) Thermostabilization of an esterase by alignment-guided focused directed evolution, Protein Eng. Des. Sel., 23, 903-909, link.

[230] Geitner, K., Rehdorf, J., Snajdrova, R., Bornscheuer, U.T. (2010) Scale-up of Baeyer-Villiger monooxygenase-catalyzed synthesis of enantiopure compounds, Appl. Microb. Biotechnol., 88, 1087-1093, link.

[229] Böttcher, D., Schmidt, M., Bornscheuer, U.T. (2010), Screens for active and stereoselective hydrolytic enzymes. Methods Mol. Biol., 668, 169-176, link.

[228] Rehdorf, J., Mihovilovic, M.D., Fraaije, M.W., Bornscheuer, U.T. (2010), Enzymatic synthesis of enantiomerically pure ß-amino ketones, ß-amino esters and ß-amino alcohols with Baeyer-Villiger-monooxygenases, Chem. Eur. J., 16, 9525-9535, link.

[227] Jochens, H., Bornscheuer, U.T. (2010), Natural diversity to guide focused directed evolution, ChemBioChem, 11, 1861-1866 link.

[226] Vorhaben, T., Böttcher, D., Jasinski, D., Brüser, V., Menyes, U., Schröder, K., Bornscheuer, U.T. (2010) Plasma-modified polypropylene as carrier for the immobilization of Candida antarctica lipase B and Pyrobaculum calidifontis esterase, ChemCatChem, 2, 992-996, link.

[225] Bassegoda, A., Nguyen, G.S., Kourist, R., Schmidt, M., Diaz, P., Bornscheuer, U.T. (2010), Rational protein design of Paenibacillus barcinonensis esterase EstA for kinetic resolution of tertiary alcohols, ChemCatChem, 2, 962-967, link.

[224] Kourist, R., Jochens, H., Bartsch, S., Kuipers, R., Padhi, S.K., Gall, M., Böttcher, D., Joosten, H.-J., Bornscheuer, U.T. (2010), The α/β-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering, ChemBioChem, 11, 1635-1643, link.

[223] Bornscheuer, U.T. (2010), The first artificial cell – arevolutionary step in synthetic biology?, Angew. Chem. Int. Ed., 49, 5228-5230, link; Bornscheuer, U.T. (2010), Die erste künstliche Zelle – Ein revolutionärer Schritt für die synthetische Biologie?, Angew. Chem., 122, 5357-5359, link.

[222] Bogdanovic, X., Hesseler, M., Palm, G.J., Bornscheuer, U.T., Hinrichs, W. (2010), Crystallization and preliminary X-ray diffraction studies on the putative haloalkane dehalogenase DPpA from Plesiocystis pacifica SIR-I, Acta Cryst. Sect. F: Struct. Biol. Cryst. Commun., 66, 828-830.

[221] Rehdorf, J., Mihovilovic, M.D., Bornscheuer, U.T. (2010), Exploiting the regioselectivity of Baeyer-Villiger-monooxygenases for the formation of ß-amino acids and ß-amino alcohols, Angew. Chem. Int. Ed., 49, 4506-4508, link; Rehdorf, J., Mihovilovic, M.D., Bornscheuer, U.T. (2010), Durch Nutzen der Regioselektivität von Baeyer-Villiger-Monooxygenasen zu ß-Aminosäuren und ß-Aminoalkoholen, Angew. Chem., 122, 4609-4611, link.

[220] Bornscheuer, U.T., Hesseler, M. (2010), Enzymatic removal of 3-monochloropropane diol (3-MCPD) and its esters from oils, Eur. J. Lipid Sci. Technol., 112, 552-556,link.

[219] Kuipers, R.K., Joosten, H.J., van Berkel, W.J.H., Leferink, N.G.H., Rooijen, E., Ittmann, E., van Zimmeren, F., Jochens, H., Bornscheuer, U., Vriend, G., Martins dos Santos, V.A.P., Schaap, P.J. (2010), 3DM: systematic analysis of heterogeneous super-family data to discover protein functionalities, Proteins, 78, 2101-2113, link.

[218] Gall, M., Kourist, R., Schmidt, M., Bornscheuer, U.T. (2010), The role of the GGG(A)X-motif on enantioselectivity of pig liver esterase towards tertiary alcohols, Biocat. Biotransform, 28, 201-208, link.

[217] Nguyen, G.S., Kourist, R., Paravidino, M., Hummel, A., Rehdorf, J., Orru, R.V.A., Hanefeld, U., Bornscheuer, U.T. (2010), An enzymatic toolbox for the kinetic resolution of 2-(pyridin-x-yl)but-3-yn-2-ols and tertiary cyanohydrins, Eur. J. Org. Chem., 2753-2758, link.

[216] Theurer, M., Fischer, P., Baro, A., Nguyen, G.S., Kourist, R., Bornscheuer, U.T., Laschat, S. (2010), Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation, Tetrahedron, 66, 3814-3823, link.

[215] Wittrup-Larsen, M., Zielinska, D.F., Martinelle, M., Hidalgo, A., Jensen, L.J., Bornscheuer, U.T., Hult, K. (2010), Suppression of water as a nucleophile in Candida antarctica lipase B catalysis, ChemBioChem, 11, 796-801, link.

[214] Schätzle, S., Höhne, M., Robins, K., Bornscheuer U.T. (2010), A conductometric method for the rapid characterization of the substrate specificity of amine-transaminases, Anal. Chem., 82, 2082-2086, link.

[213] Böttcher, D., Bornscheuer, U.T. (2010) Protein engineering of microbial enzymes, Curr. Opin. Microbiol., 13, 274-282, link.

[212] Brüsehaber, E., Schwiebs, A., Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2010), Production of pig liver esterase in batch fermentation of E. coli Origami, Appl. Microb. Biotechnol., 86, 1337-1344, link.

[211] Rzeznicka, K., Schätzle, S., Böttcher, D., Klein, J., Bornscheuer, U.T. (2010), Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterization, Appl. Microbiol. Biotechnol., 85, 1417-1425, link.

[210] Wardenga, R., Lindner, H.A., Hollmann, F., Thum, O., Bornscheuer, U.T. (2010) Increasing the synthesis/ hydrolysis ratio of aminoacylase-1 by site-directed mutagenesis, Biochimie, 92, 102-109, link.

[209] Kourist, R., Brundiek, H., Bornscheuer, U.T. (2010), Protein engineering and discovery of lipases, Eur. J. Lipid Sci. Technol., 112, 64-74, link.

[208] Fernandez-Alvaro, E., Kourist, R., Winter, J., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Jäger, K.E., Streit, W. Bornscheuer, U.T. (2010), Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases, Microb. Biotechnol., 3, 59-64, link.

2009

[207] Bornscheuer, U.T. (2009) Modern tools in the discovery and design of biocatalysts, Enzyme Engineering News Japan, 62, 5-14.

[206] Brüsehaber, E., Böttcher, D., Bornscheuer, U.T. (2009), Insights into the physiological role of pig liver esterase: Isoenzymes show differences in the demethylation of prenylated proteins, Bioorg. Med. Chem., 17, 7878-7883, link.

[205] Schließmann, A., Hidalgo, A., Berenguer, J., Bornscheuer, U.T. (2009) Increased enantioselectivity by engineering bottleneck mutants in an esterase from Pseudomonas fluorescens, ChemBioChem, 10, 2920-2923, link.

[204] Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2009), Protein engineering of carboxyl esterases by rational design and directed evolution, Protein Peptide Lett., 16, 1162-1171.

[203] Thodi, K., Barbayianni, E., Fotakopoulou, I., Bornscheuer, U.T., Constantinou-Kokotou, V., Moutevelis-Minakakis, P., Kokotos, G. (2009), Study of the removal of allyl esters by Candida antarctica lipase B (CAL-B) and pig liver esterase (PLE), J. Mol. Catal. B: Enzymatic, 61, 241-246, link.

[202] Schätzle, S., Höhne, M., Redestad, E., Robins, K., Bornscheuer U.T. (2009), A rapid and sensitive kinetic assay for characterization of omega-transaminases, Anal. Chem, 81, 8244-8248, link.

[201] Barbayianni, E., Kokotos, C.G., Bartsch, S., Drakou, C., Bornscheuer, U.T., Kokotos, G. (2009), Bacillus subtilis esterase (BS2) and its double mutant have different selectivity in the removal of carboxyl protecting groups, Adv. Synth. Catal., 351, 2325-2332, link.

[200] Adamczak, M., Bednarski, W., Bornscheuer, U.T. (2009), The application of biotechnological methods for synthesis of biodiesel, Eur. J. Lipid Sci. Technol., 111, 808-813 link.

[199] Kazlauskas, R.J., Bornscheuer U.T. (2009), Finding better protein engineering strategies, Nat. Chem. Biol., 5, 526-529, link.

[198] Royter, M., Schmidt, M., Elend, C., Höbenreich, H., Schäfer, T., Bornscheuer, U.T., Antranikian, G. (2009), Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis, Extremophiles, 13, 769-783, link.

[197] Höhne, M., Bornscheuer, U.T. (2009), Biocatalytic routes to optically active amines, ChemCatChem, 1, 42-51, link.

[196] Rehdorf, J., Lengar, A., Bornscheuer, U.T., Mihovilovic, M.D. (2009), Kinetic resolution of arylaliphatic acyclic ß-hydroxyketones by recombinant whole-cell Baeyer-Villiger monooxygenases – formation of enantiocomplementary regioisomeric esters, Bioorg. Med. Chem. Lett., 19, 3739-3743, link.

[195] Wiggers, M., Holt, J., Kourist, R., Bartsch, S., Arends, I.W.C.E., Minnaard, A.J., Bornscheuer, U.T., Hanefeld, U. (2009), Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols, J. Mol. Catal. B: Enzymatic, 60, 82-86, link.

[194] Kourist, R., Höhne, M., Bornscheuer, U.T. (2009), Protein Design: Im Spannungsfeld zwischen gerichteter Evolution und rationalem Design, Chemie in unserer Zeit, 43, 132-142, link.

[193] Bartsch, S., Bornscheuer, U.T. (2009), A single residue influences the reaction mechanism of ammonia lyases and mutases, Angew. Chem. Int. Ed., 48, 3362-3365, link; Corrigendum, Angew. Chem. Int. Ed., 49, 3860, link. Einfluss einer einzelnen Aminosäure auf den Reaktionsmechanismus von Ammonium-Lyasen und -Mutasen, Angew. Chem., 121, 3412-3415, link; Corrigendum, Angew. Chem., 122, 3951, link

[192] Jochens, H., Stiba, K., Savile, C., Fujii, R., Yu, J.-G., Gerassenkov, T., Kazlauskas, R.J., Bornscheuer, U.T. (2009), Converting an esterase into an epoxide hydrolase, Angew. Chem. Int. Ed., 48, 3532-3535, link; Umwandlung einer Esterase in eine Epoxidhydrolase, Angew. Chem., 121, 3584-3587, link.

[191] Rehdorf, J., Zimmer, C., Bornscheuer, U.T. (2009), Cloning, expression and biocatalytic investigation of 4-hydroxyacetophenone monooxygenase from Pseudomonas putida JD1, Appl. Environm. Microbiol., 75, 3106-3114, link

[190] Rachinskiy, K., Schultz, H, Boy, M., Bornscheuer, U.T., Büchs, J. (2009), Enzyme test bench: a high-throughput enzyme characterization technique including the long term stability, Biotechnol. Bioeng. 103, 302-322, link.

[189] Krause, P., Hilterhaus, L., Fieg, G., Liese, A., Bornscheuer, U.T. (2009), Chemically and enzymatically catalyzed synthesis of C6-C10 alkyl benzoates, Eur. J. Lipid Sci. Technol., 111, 194-201, link.

[188] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2009), Improving ascorbyl oleate synthesis by application of ionic liquids and water activity control by salt hydrates, Proc. Biochemistry, 44, 257-261, link.

2008

[187] Wardenga, R., Hollmann, F., Thum, O., Bornscheuer, U.T. (2008) Functional expression of porcine aminoacylase 1 in E. coli using a codon optimized synthetic gene and molecular chaperones, Appl. Microbiol. Biotechnol., 81, 721-729, link.

[186] Heinze, B., Hoven, N., O'Connell, T., Maurer, K.H., Bartsch, S., Bornscheuer, U.T. (2008) Recovery of choline oxidase activity by in vitro recombination of individual segments, Appl. Microbiol. Biotechnol., 81, 275-282, link.

[185] Kirschner, A., Bornscheuer, U.T. (2008), Directed evolution of a Baeyer-Villiger monooxygenase to enhance enantioselectivity, Appl. Microbiol. Biotechnol., 81, 465-472, link.

[184] Bornscheuer, U.T. (2008), Alteration of lipase properties by protein engineering methods, Oléagineux Corps Gras Lipides, 15, 184-188.

[183] Wittrup-Larsen, M., Bornscheuer, U.T., Hult, K. (2008), Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems, Prot. Expr. Purif., 62, 90-97, link.

[182] Kourist, R., Nguyen, G.S., Strübing, S., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Bornscheuer, U.T. (2008), Hydrolase-catalyzed stereoselective preparation of protected a,a-dialkyl-a-hydroxycarboxylic acids, Tetrahedron: Asymmetry, 19, 1839-1843, link.

[181] Hidalgo, A., Schliessmann, A., Molina, R. Hermoso, J., Bornscheuer, U.T. (2008), An one-pot, simple methodology for cassette randomization and recombination for focused directed evolution (OSCARR), Protein Des. Eng. Sel., 21, 567-576, link.

[180] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2008), Properties and biotechnological methods to produce lipids containing conjugated linoleic acid (CLA), Eur. J. Lipid Sci. Technol., 110, 491-502, link.

[179] Höhne, M., Robins, K., Bornscheuer, U.T. (2008), A protection strategy substantially enhances rate and enantioselectivity in transaminase-catalyzed kinetic resolutions, Adv. Synth. Catal., 350, 807-812, link.

[178] Brüsehaber, E., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Bornscheuer, U.T. (2008), Asymmetric synthesis of cis-3,5-diacetoxycyclopent-1-ene using metagenome derived hydrolases, Tetrahedron: Asymmetry, 19, 730-732, link.

[177] Kourist, R., Dominguez de Maria, P., Bornscheuer, U.T. (2008), Enzymatic synthesis of optically active tertiary alcohols: Expanding the biocatalytic toolbox, ChemBioChem, 9, 491-498, link.

[176] Vahl, K., Kahlert, H., Böttcher, D., Wardenga, R., Komorsky-Lovric, S., Bornscheuer, U., Scholz, F. (2008), A potential high-throughput method for the determination of lipase activity by potentiometric FIA titrations, Anal. Chem. Acta, 610, 44-49, link.

[175] Bartsch, S., Kourist, R., Bornscheuer, U.T. (2008), Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase, Angew. Chem. Int. Ed., 47, 1508-1511 link; Vollständige Umkehrung der Enantioselektivität gegen acetylierte tertiäre Alkohole durch eine Doppelmutation in einer Esterase aus Bacillus subtilis, Angew. Chem., 120, 1531-1534, link.

[174] Höhne, M., Kühl, S., Robins, K., Bornscheuer, U.T. (2008), Efficient asymmetric synthesis of chiral amines by combining transaminase and pyruvate decarboxylase, ChemBioChem, 9, 363-365, link.

[173] Völker, A., Kirschner, A., Bornscheuer, U.T., Altenbuchner, J. (2008), Functional expression, purification and characterization of the recombinant Baeyer-Villiger monooxygenase MekA from Pseudomonas veronii MEK700, Appl. Microbiol. Biotechnol., 77, 1251-1260, link.

[172] Bertram, M. Hildebrandt, P., Weiner, D.W., Patel, J. S., Bartnek, F., Hitchman, T., Bornscheuer, U.T. (2008), Characterization of lipases and esterases from metagenomes for lipid modification, J. Am. Oil Chem. Soc., 85, 47-53, link.

[171] Kourist, R., Bartsch, S., Fransson, L., Hult, K., Bornscheuer, U.T. (2008), Understanding promiscuous amidase activity of an esterase from Bacillus subtilis, ChemBioChem, 9, 67-69, link.

2007

[170] DiLorenzo, M., Hidalgo, A., Molina, R., Hermoso, J.A., Pirozzi, D., Bornscheuer, U.T. (2007), Enhancement of the stability of a prolipase from Rhizopus oryzae towards aldehydes by saturation mutagenesis, Appl. Environm. Microbiol. 73, 7291-7299, link.

[169] Hummel, A., Brüsehaber, E., Böttcher, D., Doderer, K., Trauthwein, H., Bornscheuer, U.T. (2007), Isoenzymes of pig liver esterase reveal striking differences in enantioselectivities, Angew. Chem. Int. Ed. 46, 8492-8494, link ; Hummel, A., Brüsehaber, E., Böttcher, D., Doderer, K., Trauthwein, H., Bornscheuer, U.T. (2007), Isoenzyme der Schweineleberesterase zeigen bemerkenswerte Unterschiede in ihrer Enantioselektivität, Angew. Chem. 119, 8644-8646, link.

[168] Gruber, C.C., Nestl, B.M., Gross., J., Hildebrandt, P., Bornscheuer, U.T., Faber, K., Kroutil, W. (2007), Emulation of racemase activity by employing a pair of stereocomplementary biocatalysts, Chem. Eur. J., 13, 8271-8276, link.

[167] Kourist, R., Krishna, S.H., Patel, J.S., Bartnek, F., Weiner, D.W., Hitchman, T., Bornscheuer, U.T. (2007), Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols, Org. Biomol. Chem., 5, 3310-3313, link.

[166] Brüsehaber, E., Böttcher, D., Musidlowska-Persson, A., Albrecht, D., Hecker, M., Doderer, K., Bornscheuer, U.T. (2007), Identification of pig liver esterase variants by tandem mass spectroscopy analysis and their characterization, Appl. Microbiol. Biotechnol., 76, 853-859, link.

[165] Rehdorf, J., Kirschner, A., Bornscheuer, U.T. (2007), Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440, Biotechnol. Lett., 29, 1393-1398, link.

[164] Kourist, R., Bartsch, S., Bornscheuer, U.T., (2007), Highly enantioselective synthesis of arylaliphatic tertiary alcohols using mutants of an esterase from Bacillus subtilis, Adv. Synth. Catal., 349, 1393-1398, link.

[163] Durban, M., Bornscheuer, U.T. (2007), An improved assay for the determination of phospholipase C activity, Eur. J. Lipid Sci. Technol., 109, 469-473, link.

[162] Geitner, K., Kirschner, A., Rehdorf, J., Schmidt, M., Mihovilovic, M.D., Bornscheuer, U.T. (2007), Enantioselective kinetic resolution of 3-phenyl-2-ketones using Baeyer-Villiger monooxygenases, Tetrahedron: Asymmetry, 18, 892-895, link.

[161] Kirschner, A., Altenbuchner, J., Bornscheuer, U.T. (2007), Design of a secondary alcohol degradation pathway from Pseudomonas fluorescens DSM50106 in an engineered E. coli, Appl. Microbiol. Biotechnol., 75, 1095-1101, link.

[160] Heinze, B., Kourist, R., Fransson, L., Hult, K., Bornscheuer, U.T. (2007), Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis, Protein Eng. Des. Sel., 20, 125-131, link.

[159] Bertram, M., C. Manschot-Lawrence, E. Flöter, Bornscheuer, U.T. (2007), A microtiter plate-based assay method to determine fat quality, Eur. J. Lipid Sci. Technol., 109, 180-185, link.

[158] Fotakopoulou, I., Barbayianni, E., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2007), Enzymatic removal of carboxyl protecting groups. Part III: Fast removal of the allyl and chloroethyl esters by Bacillus subtilis esterase (BS2), J. Org. Chem., 72, 782-786, link.

[157] Durban, M., Silbersack, J., Schweder, T., Schauer, F., Bornscheuer, U.T. (2007), High level expression of a recombinant phospholipase C from Bacillus cereus in B. subtilis, Appl. Microbiol. Biotechnol., 74, 634-639, link.

[156] Schmidt, M., Henke, E., Heinze, B., Kourist, R., Hidalgo, A., Bornscheuer, U.T. (2007), A versatile esterase from Bacillus subtilis: Cloning, expression, characterization, and its application in biocatalysis, Biotech. J., 2, 249-253, link.

[155] Böttcher, D., Brüsehaber, E., Doderer. K., Bornscheuer, U.T. (2007), Functional expression of the gamma-isoenzyme of pig liver carboxyl esterase in Escherichia coli, Appl. Microbiol. Biotechnol., 73, 1282-1289, link.

[154] Kirschner, A., Altenbuchner, J., Bornscheuer, U.T. (2007), Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas fluorescens DSM50106 in E.coli, Appl. Microbiol. Biotechnol., 73, 1065-1072, link.

2006

[153] Böttcher, D., Bornscheuer, U.T. (2006), High-throughput screening of activity and enantioselectivity of esterases, Nature Protocols, 1, 2340-2343, online, link.

[152] Kirschner, A., Bornscheuer, U.T. (2006), Katalytische kinetische Racematspaltung von 4-Hydroxy-2-Ketonen durch eine Baeyer-Villiger Monooxygenase, Angew. Chem., 118, 7161-7163 (german version), link; Kirschner, A., Bornscheuer, U.T. (2006), Kinetic resolution of 4-hydroxy-2-ketones by a Baeyer-Villiger monooxygenase, Angew. Chem. Int. Ed. 45, 7004-7006 (english version), link.

[151] Bellur, E., Freifeld, I., Holtz, E., Böttcher, D., Bornscheuer, U.T., Langer, P. (2006), Synthesis of (tetrahydrofuran-2-yl)acetates and (pyrrolidin-2-yl)acetates based on a 'cyclization / hydrogenation' strategy, Tetrahedron, 62, 7132-7139, link.

[150] Metzger, J.O., Bornscheuer, U.T. (2006), Lipids as renewable resources: Current state of chemical and biotechnological conversion and diversification, Appl. Microbiol. Biotechnol., 71, 13-22, link.

[149] Chaparro-Riggers, J.F., Breves, R., Maurer, K.H., Bornscheuer U.T. (2006), Modulation of infectivity in phage display as a tool to determine the substrate specificity of proteases, ChemBioChem., 7, 965-970, link.

[148] Bellur, E., Böttcher, D., Bornscheuer, U.T., Langer, P. (2006), Enantioselective synthesis of 2-alkylidenetetrahydrofurans based on a 'cyclization / enzymatic resolution' strategy, Tetrahedron: Asymmetry, 17, 892-899, link.

[147] Brandt, B., Hidalgo, A., Bornscheuer, U.T. (2006), Immobilization of enzymes in the microtiter plate scale, Biotech. J., 1, 582-587, link.

[146] Schmidt, M., Hasenpusch, D., Kähler, M., Kirchner, U., Wiggenhorn, K., Langel, W., Bornscheuer, U.T. (2006), Directed evolution of an esterase from Pseudomonas fluorescens yields a mutant with excellent enantioselectivity and activity for the kinetic resolution of a chiral building block, ChemBioChem., 7, 805-809, link.

[145] Ganske, F., Bornscheuer, U.T. (2006), Growth of Escherichia coli, Pichia pastoris and Bacillus cereus in the presence of the ionic liquids [BMIM][BF4] and [BMIM][PF6] and organic solvents, Biotechnol. Lett., 28, 465-469, link.

2005

[144] Xu, J.-H., Zhou, R., Bornscheuer, U.T. (2005), Comparison of differently modified Pseudomonas cepacia lipases in enantioselective preparation of a chiral alcohol for agrochemical use, Biocat. Biotransform., 23, 415-422, link .

[143] Di Lorenzo, M., Hidalgo, A., Haas, M., Bornscheuer, U.T. (2005), Heterologous production of functional Rhizopus oryzae lipase forms in Eschericha coli, Appl. Environm. Microbiol., 71, 8974-8977, link.

[142] Sareen, R., Bornscheuer, U.T., Mishra, P., Cloning, functional expression and characterization of an alkaline, organic solvent tolerant protease from Bacillus licheniformis RSP-09-37, Biotechnol. Lett., 27, 1901-1907, link.

[141] Poessl, T.M., Kosjek, B., Ellmer, U., Gruber, C.C., Edegger, K., Faber, K. Hildebrandt, P., Bornscheuer, U.T., Kroutil, W., Stereo-complementary non-racemic halohydrins via biocatalytic hydrogen transfer-reduction of halo-ketones and one-pot cascade-reaction to enantiopure epoxide, Adv. Synth. Catal., 345, 1827-1834, link.

[140] Ödman, P., Wessjohann, L.A., Bornscheuer, U.T. (2005), Chemoenzymatic dynamic kinetic resolution of acyloins, J. Org. Chem., 70, 9551-9555, link.

[139] Barbayianni, E., Fotakopoulou, I., Schmidt, M., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2005), Enzymatic removal of carboxyl protecting groups. Cleavage of the benzyl- and methyl moieties, J. Org. Chem., 70, 8730-8733, link.

[138] Ganske, F., Bornscheuer, U.T. (2005), Optimization of lipase-catalyzed glucose fatty acid ester synthesis in a two-phase systems containing ionic liquids and t-BuOH, J. Mol. Catal. B: Enzym., 36, 40-42, link.

[137] Bornscheuer, U.T., Rodriguez Ordonez, G., Hidalgo, A., Gollin, A., Lyon, J., Hitchman, T.S., Weiner, D.P. (2005), Selectivity of lipases and esterases towards phenol esters, J. Mol. Catal. B: Enzymatic, 36, 8-13, link.

[136] Buchholz, K., Bornscheuer, U.T. (2005), Highlights in biocatalysis – Historical landmarks and current trends, Eng. Life Sci., 5, 309-323, link.

[135] Bornscheuer, U.T. (2005), Deep-sea mining for unique biocatalysts, Chem. Biol., 12, 859-860, link.

[134] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2005), Synthesis of ascorbyloleate by immobilized Candida antarctica lipases, Process Biochemistry, 40, 3177-3180, link.

[133] Bornscheuer U.T. (2005), White biotechnology for lipids, fats and oils, Eur. J. Lipid Sci. Technol., 107, 445-446, link.

[132] Chaparro-Riggers, J.F., Breves, R., Michels, A., Maurer, K.H., Bornscheuer U.T. (2005), A GFP-based assay for the determination of hydrolytic activity and substrate specificity of subtilisins under washing conditions, J. Mol. Catal. B: Enzymatic, 35, 74-77, link.

[131] Ganske, F., Bornscheuer, U.T. (2005), Lipase-catalyzed glucose fatty acid ester synthesis in ionic liquids, Org. Lett., 7, 3097-3098, link.

[130] Strübing, D., Kirschner, A., Neumann, H., Hübner, S., Klaus, S., Bornscheuer, U.T., Beller, M. (2005), Synthesis of enantiomerically pure cyclohex-2-en-1-ols: Development of novel multicomponent reactions, Chem. Eur. J., 11, 4210-4218, link.

[129] Schmidt, M., Bornscheuer, U.T. (2005), High-throughput assays for lipases and esterases, Biomolecular Eng., 22, 51-56, link.

[128] Robertson, D.E., Bornscheuer, U.T. (2005), Biocatalysis and biotransformation. New technologies, enzymes and challenges, Curr. Opin. Chem. Biol., 9, 164-165, link.

[127] Schmidt, M., Barbayianni, E., Fotakopoulou, I., Höhne, M., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2005), Enzymatic Removal of Carboxyl Protecting Groups. Part I: Cleavage of the tert-Butyl Moiety, J. Org. Chem., 70, 3737-3040, available online as ASAP article, link.

2004

[126] Soumanou, M., Aleodjrodo, P., Bornscheuer, U.T. (2004), Activity and stability of immobilized lipases in lipase-catalyzed modification of peanut oil, Oleagineux, Corps Gras, Lipides, 11, 464-468.

[125] Sareen, R., Bornscheuer, U.T., Mishra, P. (2004), Synthesis of Kyotorphin precursor by an organic solvent–stable protease from Bacillus licheniformis RSP-09-37, J. Mol. Catal. B: Enzym., 32, 1-5, link.

[124] Bornscheuer, U.T. (2004), High-throughput-screening systems for hydrolases, Eng. Life. Sci., 4, 539-542, link.

[123] Bornscheuer, U.T., Kazlauskas, R.J. (2004), Catalytic promiscuity in biocatalysis: Using old enzymes to form new bonds and follow new pathways, Angew. Chem. Int. Ed. Engl., 42, 6032-6040, link; Untreue Enzyme in der Biokatalyse: Mit alten Enzymen zu neuen Bindungen und Synthesewegen, Angew. Chem., 116, 6156-6165, link.

[122] Wongsakul, S., Bornscheuer, U.T. Kittikun, H. (2004), Lipase-catalyzed acidolysis and Phospholipase D-catalyzed transphosphatidylation of phosphatidylcholine, Eur. J. Lipid Sci. Technol., 106, 665-670, link.

[121] Scheid, G., Ruijter, E., Konarzycka-Bessler, M., Bornscheuer, U.T., Wessjohann, L.A. (2004), Synthesis and resolution of a key building block for epothilones – a comparison of asymmetric synthesis, chemical, and enzymatic resolution, Tetrahedron: Asymmetry, 15, 2861-2869, link.

[120] Kirschner, A., Langer, P., Bornscheuer, U.T. (2004), Lipase-catalyzed highly enantioselective kinetic resolution of alpha-hydroxy butenolides, Tetrahedron: Asymmetry, 15, 2871-2874, link.

[119] Bornscheuer, U.T. (2004), Finding enzymatic gold on silver surfaces, Nat. Biotechnol., 22, 1098-1099, pdf, link .

[118] Sareen, R., Bornscheuer, U.T., Mishra, P. (2004), A microtiter plate assay for the determination of the synthetic activity of protease, Anal. Biochem., 333, 193-195, link.

[117] Schmidt, M., Baumann, M., Henke, E., Konarzycka-Bessler, M., Bornscheuer U.T. (2004), Directed evolution of lipases and esterases, Methods Enzymol., 388, 199-207.

[116] Wongsakul, S., Kittikun, A., Bornscheuer, U.T. (2004), Lipase-catalyzed synthesis of structured triglycerides from 1,3-diglycerides, J. Am. Oil Chem. Soc., 81, 151-155.

[115] Schönfeld, D.L., Bornscheuer, U.T. (2004), A polarimetric assay for the medium-throughput determination of amino acid racemase activity, Anal. Chem., 76, 1184-1188, link.

[114] Wessjohann, L.A., Scheid, G., Orru, R.V.A., Kuit, W., Ruijter, E., Henke, E., Bornscheuer, U.T. (2004), A new route to protected acyloins and their enzymatic resolution with lipases, Eur. J. Org. Chem., 1063-1074, link.

2003

[113] Musidlowska-Persson, A., Bornscheuer, U.T. (2003), Recombinant porcine intestinal carboxyl esterase: Cloning from the pig liver esterase gene by site-directed mutagenesis, functional expression and characterization, Protein Eng., 16, 1139-1145, link.

[112] Soumanou, M.M., Bornscheuer, U.T. (2003), Lipase-catalyzed alcoholysis of vegetable oils, Eur. J. Lipid Sci. Technol., 105, 656-660, link.

[111] Durban, M., Bornscheuer, U.T. (2003), An assay system for the detection of Phospholipase C activity, Eur. J. Lipid Sci. Technol., 105, 633-637, link.

[110] Ganske, F., Meyer, H.H., Deutz, H., Bornscheuer, U.T. (2003), Enzyme-catalyzed hydrolysis of 18-methyl eicosanoic acid-cysteine thioester, Eur. J. Lipid Sci. Technol., 105, 627-632, link.

[109] Bornscheuer, U.T., Musidlowska-Persson, A., Böttcher, D., Liebeton, K., Lorenz, P., Eck, J., Zinke, H., Schleper, C., Langer, P., Trauthwein, H., Karau, A., Buchholz, S. (2003), Esterasen und Lipasen aus kultivierten und nicht-kultivierten Mikroorganismen, Transkript, 9 (10), 81-84.

[108] Kähler, M., Rieks, A., Kirchner, U., Wiggenhorn, K., Bornscheuer, U.T., Schmidt, M., Eisner, A., Petersen, W., Ellerbrock, F., Bollow, S. (2003), Biokatalytische Synthese enantiomerenreiner building blocks, Transkript, 9 (10), 78-80.

[107] Bornscheuer, U.T. (2003), Immobilizing enzymes: how to create more suitable biocatalysts, Angew. Chem. Int. Ed. Engl. 42, 3336-3337, link; Enzymimmobilisierung: ein Weg zu verbesserten Biokatalysatoren, Angew. Chem., 115, 3458-3459, link.

[106] Soumanou, M.M., Bornscheuer, U.T. (2003), Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil to Biodiesel using immobilized lipases, Enzyme Microb. Technol., 33, 97-103, link.

[105] Henke, E., Bornscheuer, U.T., Schmid, R.D., Pleiss, J. (2003), The molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases, Chem. Bio. Chem., 4, 485-493, link.

[104] Musidlowska-Persson, A., Bornscheuer, U.T. (2003), Site-directed mutagenesis of recombinant pig liver esterase
yields mutants with altered enantioselectivity, Tetrahedron: Asymmetry, 14, 1341-1344, link.

[103] Horsman, G.P., Liu, A.F.M., Henke, E., Bornscheuer, U.T., Kazlauskas, R.J. (2003), Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase toward methyl 3-Bromo-2-methylpropanoate and ethyl 3-phenylbutyrate, Chem. Eur. J., 9, 1933-1939, link.

[102] Konarzycka-Bessler, M. Bornscheuer, U.T. (2003), A high-throughput-screening method for the determination of the synthetic activity of hydrolases, Angew. Chem. Int. Ed., 42, 1418-1420, link; Eine Hochdurchsatz-Screeningmethode zur Bestimmung der Syntheseaktivität von Hydrolasen, Angew. Chem., 115, 1449-1451, link.

[101] Persson, M.M., Bornscheuer, U.T. (2003), Increased stability of an esterase from Bacillus subtilis in ionic liquids as compared to organic solvents, J. Mol. Catal. B: Enzymatic, 22, 21-27, link.

[100] Wongsakul, S., Prasertsan, P., Bornscheuer, U.T., H-Kittikun, A. (2003), Synthesis of 2-monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases, Eur. J. Lipid Sci. Technol., 105, 68–73, link.

[99] Henke, E., Bornscheuer, U.T. (2003), Fluorophoric assay for the high-throughput determination of amidase activity, Anal. Chem., 75, 255-260 (in press (DOI), link.

2002
[98] Hari Krishna, S., Persson, M.M., Bornscheuer, U.T. (2002), Enantioselective transesterification of a tertiary alcohol by Lipase A from Candida antarctica, Tetrahedron: Asymmetry, 13, 2693-2696.

[97] Bornscheuer, U.T. (2002), Methods to increase enantioselectivity of lipases and esterases, Curr. Opin. Biotechnol., 13, 543-547.

[96] Vorlová, Bornscheuer, U.T., Gatfield, I., Hilmer, J.M., Bertram, H.J., Schmid, R.D., (2002), Enantioselective hydrolysis of D,L-menthyl benzoate to L-menthol by recombinant Candida rugosa lipases, Adv. Synth. Catal., 344, 1152-1155, link.

[95] Henke, E., Bornscheuer, U.T., (2002), Esterases from Bacillus subtilis and Bacillus stearothermophilus share high sequence homology but differ substantially in their properties, Appl. Microbiol. Biotechnol., 60, 320-326, link.

[94] Musidlowska-Persson, A., Bornscheuer, U.T. (2002), Substrate specificity of the gamma-isoenzyme of recombinant pig liver esterase towards acetates of secondary alcohols, J. Mol. Catal. B: Enzymatic, 19-20C, 129-133.

[93] Bornscheuer, U.T., Bessler, C., Srinivas, R., Krishna, S.H. (2002), Optimizing lipases and related enzymes for efficient application, Trends Biotechnol., 20, 433-437.

[92] Henke, E., Pleiss, J., Bornscheuer, U.T. (2002), Activity of lipases and esterases towards tertiary alcohols: Insights into structure-function relationships, Angew. Chem. Int. Ed., 41, 3211-3213, link. Aktivität von Lipasen und Esterasen gegenüber tertiären Alkoholen: Neue Einblicke in Struktur-Funktions-Beziehungen, Angew. Chem., 114, 3338-3341, link,supporting information: pdf.

[91] Hildebrandt, P., Musidlowska-Persson, A., Bornscheuer, U.T., Altenbuchner, J. (2002), Cloning, functional expression and biochemical characterization of a stereoselective dehydrogenase from Pseudomonas fluorescens DSM50106, Appl. Microbiol. Biotechnol., 59, 483-487.

[90] Bornscheuer, U.T. (2002), Microbial carboxyl esterases - Classification, properties and application in biocatalysis, FEMS Microbiol. Rev. 26, 73-81.

[89] Henke, E., Baumann, M., Musidlowska-Persson, A., Bornscheuer, U.T. (2002), Innovative Methoden zur Erzeugung stereoselektiver Esterasen, BIOforum 4, 218-219.

[88] Yan, Y., Bornscheuer, U.T., Schmid, R.D. (2002), Efficient water removal in lipase-catalyzed esterifications using a low-boiling-point azeotrope, Biotechnol. Bioeng. 78, 31-34, link.

2001
[87] Baumann, M., Stürmer, R., Bornscheuer, U.T. (2001), A high-throughput-screening method for the identification of active and enantioselective hydrolases, Angew. Chem. Int. Ed., 40, 4201-4204, link. Eine Hochdurchsatz-Screening-Methode zur Identifizierung aktiver und enantioselektiver Hydrolasen, Angew. Chem. 113, 4329-4333, link. (HOT PAPER).

[86] Bornscheuer, U.T. (2001), Lipase-catalyzed synthesis of structured triglycerides, Lipid Technology, 7, 105-107.

[85] Yan, Y., Stadler, G., Lutz-Wahl, S., Otto, R.T., Reuss, M., Bornscheuer, U.T., Schmid, R.D. (2001), Regioselective glucose ester synthesis on preparative scale, Eur. J. Lipid Sci. Technol., 103, 583-587, link.

[84] Lange, S., Musidlowska,A., Schmidt-Dannert, C., Schmitt, J., Bornscheuer, U.T. (2001), Cloning,functional expression and characterization of recombinant pig liver esterase, ChemBioChem.,2, 576-582, link.

[83] Musidlowska, A., Lange, S., Bornscheuer, U.T. (2001), Via Overexpression in the yeast Pichia pastoris to enhanced enantioselectivity: New aspects in the application of pig liver esterases, Angew. Chem. Int. Ed., 40, 2851-2853, link. Durch Überexpression in der Hefe Pichia pastoris zu erhöhter Enantioselektivität: Neue Aspekte in der Anwendung von Schweineleberesterase, Angew. Chem., 113, 2934-2936, link.

[82] Hildebrandt, P., Riermeier, T., Altenbuchner, J., Bornscheuer, U.T. (2001), Efficient resolution of prostereogenic arylaliphatic ketones using a recombinant alcohol dehydrogenase from Pseudomonas fluorescens DSM50106 expressed in E. coli, Tetrahedron: Asymmetry, 12, 1207-1210.

[81] Bornscheuer, U.T. (2001), Directed evolution of enzymes for biocatalytic applications, Biocatal. Biotransform., 19, 84-96.

[80] Liu, A. M. F., Somers, N. A., Kazlauskas, R. J., Brush, T. S., Zocher, F., Enzelberger, M. M., Bornscheuer, U. T., Horsman, G. P., Mezzetti, A., Schmidt-Dannert, C., Schmid, R. D. (2001), Mapping the substrate selectivity of new hydrolases using colorimetric screening:lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes, Tetrahedron: Asymmetry, 12, 545-556.

[79] Bornscheuer,U. T.,Pohl, M. (2001), Improved biocatalysts by directed evolution and rationalprotein design, Curr. Opin. Chem. Biol., 5, 137-142 (pdf). With permission from Elsevier Science, single copies of the article may be downloaded and printed for the reader's personal research and study here.

[78] Yan, Y., Bornscheuer, U. T., Stadler, G., Lutz-Wahl, S., Reuss, M., Schmid, R. D. (2001), Production of sugar fatty acid esters by enzymatic esterification in a stirred-tankmembrane reactor: Optimization of parameters by response surface methodology, J. Am. Oil Chem. Soc. 78, 147-152.

2000
[77] Yan, Y., Bornscheuer, U.T., Stadler, G., Reuss, R., Schmid, R.D. (2000), Enzymatic productionof biosurfactant 6-O-glucose fatty acid monoester, J. Deterg. Cosmetics(in Chinese), 23, 71-77.

[76] Baumann, M., Hauer,B.H., Bornscheuer, U.T. (2000), Rapid screening of hydrolases for the enantioselective conversion of "difficult-to-resolve substrates", Tetrahedron: Asymmetry,11, 4781-4790, supporting information (pdf-file).

[75] Bornscheuer, U. T. (2000), Directed evolution of enzymes, Chim. Oggi/ Chem. Today, 18(9), 65-67.

[74] Stander, M. A., Bornscheuer, U. T., Henke, E., Steyn, P. S. (2000), Screening of commercial hydrolases for the degradation of Ochratoxin A, J. Agric. Food Chem., 48, 5736-5739, link.

[73] Peters, H., Schmidt-Dannert, C., Cao, L., Bornscheuer, U.T., Schmid, R.D. (2000), Purification and reconstitution of an integral membrane protein, the photoreaction center of Rhodobacter sphaeroides, using synthetic sugar esters, Biotechniques 28, 1214-1219.

[72] Henke, E., Schuster, S., Yang, H., Bornscheuer, U.T., (2000), Lipase-catalyzed resolution of Ibuprofen, Chem. Month. 131, 633-638.

[71] Otto, R., Scheib, H., Bornscheuer, U.T., Pleiss, J., Syldatk, C., Schmid, R.D. (2000), Substrate specificity of lipase B from Candida antarctica in the synthesis of arlyaliphatic glycolipids, J. Mol. Catal. B: Enzymatic 8,210-211.

1999
[70] Yan, Y., Bornscheuer, U.T., Schmid, R.D. (1999), Lipase-catalyzed synthesis of vitamin C fattyacid esters, Biotechnol. Lett.21, 1051-1054.

[69] Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Wohlleben, W., Schmid, R.D. (1999),Epoxide hydrolase activity of Streptomyces strains, J. Biotechnol.77 ,287-292.

[68] Wolfangel, P., Meyer, H.H., Bornscheuer, U.T., Müller, K. (1999), Solid state NMR and FTIR studies on bilayer membranes from 1,2-dioctadec-(14-ynoyl)-sn-glycero-3-phosphatidylcholine, Biochim.Biophys. Acta 1420, 121-138.

[67] Henke, E., Bornscheuer, U.T. (1999), Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay, Biol. Chem. 380, 1029-1033.

[66] Yan, Y., Bornscheuer, U.T., Cao, L., Schmid, R.D. (1999), Lipase-catalyzed solid phase synthesis of sugar fatty acid esters. Removal of by-products by azeotropic distillation, Enzyme Microb. Technol. 25, 725-728.

[65] Bornscheuer, U.T., Altenbuchner, J., Meyer., H.H. (1999), Directed evolution of an esterase: Screening of enzyme libraries based on pH-indicators and a growth assay, Bioorg.Med. Chem.7, 2169-2173.

[64] Schmid, U., Bornscheuer, U.T., Soumanou, M.M., McNeill, G.P., Schmid, R.D. (1999), Highly-selective synthesis of 1,3-oleyl-2-palmitoyl-glycerol by lipase catalysis, Biotechnol. Bioeng. 64, 678-684.

[63] Ji, H.-S., McNiven,S., Yano, K., Ikebukuro, K., Bornscheuer, U.T., Schmid, R.D., Karube, I.(1999), Highly sensitive trilayer piezoelectric odor sensor, Anal. Chim.Acta 387, 39-45.

[62] Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Schmid, R.D. (1999), A colorimetric assay suitable for screening epoxide hydrolase activity, Anal. Chim. Acta 391, 345-351.

[61] Khalameyzer, V., Bornscheuer, U.T. (1999), Overexpression and characterization of an esterase from Streptomyces diastatochromogenes, Biotechnol. Lett.21, 101-104.

[60] Yang, H., Henke, E., Bornscheuer, U.T. (1999), Highly efficient double enantioselection by lipase-catalyzed transesterification of (R,S)-carboxylic acid vinyl esters with ( R,S)-1-phenylethanol,Tetrahedron:Asymmetry10, 957-960.

[59] Yang, H., Henke, E., Bornscheuer, U.T. (1999), The use of vinyl esters significantly enhancedenantioselectivities and reaction rates in lipase-catalyzed resolutions of arlyaliphatic carboxylic acids, J. Org. Chem. 64, 1709-1712.

[58] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1999), Lipase-catalyzed solid-phase synthesis of sugar esters. Influence of immobilization on productivity and stability of the enzyme, J. Mol. Catal. B: Enzymatic 6, 279-285.

[57] Khalameyzer, V., Fischer, I., Bornscheuer, U.T., Altenbuchner, J. (1999), Screening, nucleotide sequence and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity toward lactones, Appl. Environm. Microbiol. 65,477-482.

[56] Minning, S., Weiss,A., Bornscheuer, U.T., Schmid, R.D. (1999), Determination of peracid and putative enzymatic peracid formation by an easy colorimetric assay, Anal.Chim.Acta 378, 295-300.

[55] Soumanou, M.M., Bornscheuer, U.T., Schmid, U., Schmid, R.D. (1999), Crucial role of support and water activity on the lipase-catalyzed synthesis of structured triglycerides, Biocatal. Biotransform. 16, 443-459.

1998
[54] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1998), Lipase-catalyzed solid phase synthesis of sugar esters, IV: Selectivity of lipases towards primary and secondary hydroxyl groups in carbohydrates, Biocatal. Biotransform. 16, 249-257.

[53] Bornscheuer, U.T. (1998), Directed evolution of enzymes, Angew. Chem. / Angew. Chem. Int. Ed. Engl. 110 / 37, 3285-3288 / 3105-3108.

[52] Schmid, U., Bornscheuer, U.T., Soumanou, M.M., McNeill, G.P., Schmid, R.D. (1998), Optimization of the reaction conditions in the lipase-catalyzed synthesis of structured triglycerides, J. Am. Oil Chem. Soc. 75, 1527-1531.

[51] Otto, R.T., Bornscheuer, U.T., Scheib, H., Pleiss, J., Syldatk, C., Schmid, R.D. (1998), Lipasecatalyzed esterification of unusual substrates: Synthesis of glucuronic acid and ascorbic acid (vitamin C) esters, Biotechnol. Lett. 20,1091-1094.

[50] Takahashi, S., Ueda, M., Atomi, H., Beer, H.D., Bornscheuer, U.T., Schmid, R.D., Tanaka, A. (1998), Extracellular production of active Rhizopus oryzae lipase by Saccharomyces cerevisiae, J. Ferment. Bioeng. 86, 164-168.

[49] Otto, R., Bornscheuer, U.T., Syldatk, C., Schmid, R.D. (1998), Lipase-catalyzed synthesis of arylaliphatic esters of b-D(+)-glucose, alkyl- and arylglucosides and characterizationof their surfactant properties, J. Biotechnol. 64, 231-237.

[48] Beer, H.D., Bornscheuer, U.T., McCarthy, J.E.G., Schmid, R.D. (1998), Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae, Biochim.Biophys. Acta 1399, 173-180.

[47] Bornscheuer, U., Heinz, E. (1998), Toward new fats, oils through biotechnology, Inform 9, 485-488.

[46] Wagegg, T., Enzelberger, M.M., Bornscheuer, U.T., Schmid, R.D. (1998), The use of methoxy acetoxyesters significantly enhances reaction rates in the lipase-catalyzed preparation of optical pure 1-(4-chloro-phenyl) ethyl amines, J. Biotechnol. 61,75-78.

[45] Enzelberger, M.M., Zocher, F., Schmidt-Dannert, C., Bornscheuer, U.T., Schmid, R.D., Hauer, B., Eipel, H. (1998), Singularization of enzyme mutants by use of a cell sorter andgreen fluorescent protein, BIOforum 4, 192-194.

[44] Otto, R.T., Bornscheuer, U.T., Syldatk, C., Schmid, R.D. (1998), Synthesis of aromatic n-alkyl-glucoside esters in a coupled ß-glucosidase and lipase reaction, Biotechnol. Lett. 20, 437-440.

[43] Soumanou, M.M., Bornscheuer, U.T., Schmid, R.D. (1998), Two-step enzymatic reaction for the synthesisof pure structured triglycerides, J. Am. Oil Chem. Soc. 75,703-710.

[42] Soumanou, M.M., Bornscheuer, U.T., Schmid, U., Schmid, R.D. (1998), Synthesis of structured triglycerides by lipase catalysis, Fett/Lipid 100, 156-160.

[41] Krebsfänger, N., Zocher, F., Altenbuchner, J., Bornscheuer, U.T. (1998), Characterization, and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens, Enzyme Microb. Technol. 21, 641-646.

[40] Yano, K., Bornscheuer, U.T., Schmid, R.D., Yoshitake, H., Ikebukuro, K., Yokoyama, K., Masuda,Y., Karube, I. (1998), Development of odorant sensor using polymer-coatedquartz crystals immobilized with unusual lipids, Biosensors Bioelectronics 13,397-405.

[39] Zocher, F., Krebsfänger, N., Yoo, O.J., Bornscheuer, U.T. (1998), Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens, J. Mol. Catal. B, Enzymatic 5, 187-190.

[38] Bornscheuer, U.T., Altenbuchner, J., Meyer, H.H. (1998), Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of Epothilones, Biotechnol. Bioeng. 58, 554-559, link.

[37] Krebsfänger, N., Schierholz, K., Bornscheuer, U.T. (1998), Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens towards alcohols and carboxylic acids, J. Biotechnol. 60, 105-111.

[36] Bornscheuer, U.T., Enzelberger, M.M., Altenbuchner, J., Meyer, H.H. (1998), Mutator strain Epicuriancoli XL1-red generates esterase variants, Strategies 11, 16-17.

[35] Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D., Schwarz, D. (1998), Branched phosphatidylcholines stimulate activity of cytochrome P450SCC (CYP11A1)in phospholipid vesicles by enhancing cholesterol binding, membrane incorporation, and protein exchange, J. Biol. Chem. 273, 1380-1286.

1997
[34] Yano, K., Yoshitake, H., Bornscheuer, U.T., Schmid, R.D., Ikebukuro, K., Yokoyama, K., Masuda, Y., Karube, I. (1997), Development of a chemical vapor sensor using piezoelectric quartz crystals coated with unusual lipids, Anal. Chim. Acta 340 ,41-48.

[33] Thude, S., Shukun, L., Said, M.B., Bornscheuer, U.T. (1997), Lipase-catalyzed synthesis of monoacylglycerides by glycerolysis of campher tree seed oil and cocoa-butter, Fett/Lipid 99,246-250.

[32] Blüher, A., Grube, A., Bornscheuer, U., Banik, G. (1997), A reappraisal of the enzyme lipase for removing drying-oil stains on paper, Paper Conservator 21 ,37-47.

[31] Soumanou, M.M., Bornscheuer, U.T., Menge, U., Schmid, R.D. (1997), Synthesis of structured triglycerides from peanut oil with immobilized lipase, J. Am. Oil Chem. Soc. 74,427-433.

[30] Cao, L., Fischer, A., Bornscheuer, U.T., Schmid, R.D. (1997), Lipase-catalyzed solid phase preparation of sugar fatty acid esters, Biocatal. Biotransform. 14, 269-283.

[29] Schwarz, D., Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Possible involvement of nonbilayer lipids in the stimulation of the activity ofcytochrome P450SCC (CYP11A1) and its propensity to induce vesicle aggregation,Chem. Phys. Lipids 85, 91-99.

[28] Pisch, S., Bornscheuer, U., Meyer, H.H., Schmid, R.D. (1997), Properties of unusual phospholipids IV: Chemoenzymatic synthesis of phospholipids bearing acetylenic fatty acids, Tetrahedron 53, 14627-14634.

[27] Negelmann, L., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Properties of unusual phospholipids III: Synthesis, monolayer investigations and DSC studies of hydroxy octadeca(e)noic acids and diacylglycerophosphocholines derived therefrom, Chem. Phys. Lipids 90, 117-134.

[26] Schwarz, D., Kisselev, P., Pfeil, W., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Evidencethat nonbilayer phase propensity of the membrane is important for the sidechain cleavage activity of cytochrome P450SCC (CYP11A1), Biochemistry 36,14262-14270.

[25] Enzelberger, M.M., Bornscheuer, U.T., Gatfield, I., Schmid, R.D. (1997), Lipase-catalysed resolution of g-and d -lactones, J. Biotechnol. 56, 129-133.

1996
[24] Gaziola, L., Bornscheuer, U., Schmid, R.D. (1996), A rapid and effective separation of enantiomers of glycerol derivatives by gas chromatography and their lipase-catalyzed biotransformation, Enantiomer1, 49-54.

[23] Wünsche, K., Schwaneberg, U., Bornscheuer, U.T., Meyer, H.H. (1996), Chemoenzymic route to b-blockers via 3-hydroxy esters, Tetrahedron: Asymmetry 7, 2017-2022.

[22] Bornscheuer, U., Gaziola, L., Schmid, R.D. (1996), Application of vinyl esters for the lipase-catalyzed high-yield synthesis of monoacylglycerols, Ann. N.Y. Acad. Sci. 799,757-761.

[21] Lampe, T.F.J., Hoffmann, H.M.R., Bornscheuer, U.T. (1996), Lipase mediated desymmetrization of meso 2,6- di(acetoxymethyl)tetrahydropyran-4-one derivatives. An innovative route to enantiopure 2,4,6-trifunctionalized C-glycosides, Tetrahedron:Asymmetry 7, 2889-2900.

[20] Bornscheuer, U., Capewell, A., Wendel, V., Scheper, T. (1996), Online determination of the conversion in a lipase-catalyzed kinetic resolution in supercritical carbon dioxide, J. Biotechnol. 46, 139-143.

[19] Heidt, M., Bornscheuer, U., Schmid, R.D. (1996), Studies on the enantioselectivity in the lipase-catalyzed synthesis of monoacylglycerols from the isopropylidene glycerol, Biotechnol. Tech. 10, 25-30.

[18] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1996), Lipase-catalyzed solid-phase synthesis of sugar esters, Fett/Lipid 98, 332-335.

[17] Capewell, A., Wendel, V., Bornscheuer, U., Meyer, H.H., Scheper, T. (1996), Lipase-catalyzed kinetic resolution of 3-hydroxy esters in organic solvents and supercritical carbon dioxide, Enzyme Microb. Technol. 19, 181-186.

1995
[16] Bornscheuer, U., Herar, A., Capewell, A., Wendel, V., Kreye, L., Scheper, T., Voß, E., Wünsche, K., Meyer, H.H. (1995), Lipase-catalyzed kinetic resolution of 3-hydroxyesters: Optimization, batch, and continuous reactions, Ann. N.Y. Acad.Sci.750, 215-221.

[15] Bornscheuer, U.T., Yamane, T. (1995), Fatty acid vinyl esters as acylating agents: a new method for the enzymic synthesis of monoacylglycerols, J. Am. Oil Chem. Soc.72 ,193-197.

[14] Bornscheuer, U.T. (1995), Lipase-catalyzed syntheses of monoacylglycerols, Enzyme Microb. Technol. 17,578-586.

[13] Bornscheuer, U. (1995), Lipase-catalyzed synthesis of monoglycerides, Fat Sci. Technol. 97,241-249.

[12] Ballesteros, A., Bornscheuer, U., Capewell, A., Combes, D., Condoret, J.-S., König, K., Kolisis, F.N., Marty, A., Menge, U., Scheper, T., Stamatis, H., Xenakis, A. (1995), Review article: Enzymes in non-conventional phases, Biocatal. Biotransform. 13, 1-42.

[11] Heß, R., Bornscheuer, U., Capewell, A., Scheper, T. (1995), Lipase-catalyzed synthesis of monostearoylglycerol in organic solvents from 1,2-O-isopropylidene glycerol, Enzyme Microb. Technol. 17, 725-728.

1994
[10] Bornscheuer, U., Stamatis, H., Xenakis, A., Yamane, T., Kolisis, F.N. (1994), A comparison of different strategies for lipase-catalyzed synthesis of partial glycerides, Biotechnol. Lett. 16, 697-702.

[9] Bornscheuer, U.T., Yamane, T. (1994), Activity and stability of lipase in the solid-phase glycerolysis of triolein, Enzyme Microb. Technol. 16, 864-869.

[8] Bornscheuer, U., Reif, O.-W., Lausch, R., Freitag, R., Scheper, T., Kolisis, F.N., Menge, U. (1994), Lipase of Pseudomonas cepacia for biotechnological purposes: purification, crystallization and characterization, Biochim. Biophys. Acta 1201, 55-60.

[7] Kreye, L., Herar, A., Bornscheuer, U., Scheper, T. (1994), Enzymic conversions in organic solvents in batch and continuous systems, Fat Sci. Technol. 96, 246-251.

1993
[6] Stamatis, H., Xenakis, A., Bornscheuer, U., Scheper, T., Menge, U., Kolisis, F.N. (1993), Pseudomonas cepacia lipase: esterification reactionsin AOT microemulsion systems, Biotechnol. Lett. 15, 703-708.

[5] Bornscheuer, U., Herar, A., Kreye, L., Wendel, V., Capewell, A., Meyer, H.H., Scheper, T., Kolisis, F.N. (1993), Factors affecting the lipase catalyzed transesterification reactions of 3-hydroxy esters in organic solvents, Tetrahedron:Asymmetry 4, 1007-1016.

1992
[4] Capewell, A., Bornscheuer, U., Herar, A., Scheper, T., Meyer, H.H., Kolisis, F. (1992), A comparison of enzymic reactions in aqueous, organic and supercritical phases, DECHEMA Biotechnol. Conf. 5, 57-60.

[3] Bornscheuer, U., Schapöhler, S., Scheper, T., Schügerl, K., König, W.A. (1992), Application of enantioselective capillary gas chromatography in lipase-catalyzed transesterification reactions in organic media, J. Chromatogr. 606, 288-290.

[2] Bornscheuer, U., Capewell, A., Scheper, T., Meyer, H.H. (1992), A comparison of enzymic reactions in aqueous, organic, and supercritical phases, Ann. N.Y. Acad. Sci. 672, 336-342.

1991
[1] Bornscheuer, U., Schapöhler, S., Scheper, T., Schügerl, K. (1991), Influences of reaction conditions on the enantioselective transesterification using Pseudomonas cepacia lipase, Tetrahedron: Asymmetry 2, 1011-1014.

 

 

 

 

 

 

 

 

News

We organize two conferences in 2012 at our Institute:

Aug. 29-31st 2012:
Protein Engineering for Biocatalysis

Sept. 19-21st 2012:
Biocatalysis in Lipid Modification

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Our publication made the cover of Nature Chem. Biol, Nov. 2010:

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Author profile:

An author profile about Uwe Bornscheuer has been published in Angew. Chem. Int. Ed.

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Flyer available:

English version (pdf)

German version (pdf)

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Cluster Partner:

Partner in the 'White Biotechnology' cluster 'Biokatalyse2021' funded by the BMBF.

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Contact
Prof. Dr. Uwe Bornscheuer
Institute of Biochemistry
Dept. of Biotechnology & Enzyme Catalysis
Felix-Hausdorff-Str. 4
D-17487 Greifswald/ Germany

Phone:
+49 (0)3834-86-4367
+49 (0)3834-86-4391 (Secr.)
Fax:
+49 (0)3834-86-794367
E-Mail:
uwe.bornscheuer

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