Prof. Dr. Uwe T. Bornscheuer
Selected Recent Publications
Rehdorf, J., Mihovilovic, M.D., Bornscheuer, U.T. (2010), Exploiting regioselectivity of Baeyer-Villiger-monooxygenases – a route to the formation of ß-amino acids and ß-amino alcohols, Angew. Chem. Int. Ed., accepted.
Kazlauskas, R.J., Bornscheuer U.T. (2009), Finding better protein engineering strategies, Nat. Chem. Biol., 5, 526-529, link.
Bartsch, S., Bornscheuer, U.T. (2009), A single residue influences the reaction mechanism of ammonia lyases and mutases, Angew. Chem. Int. Ed., 48, 3362-3365, online.
Jochens, H., Stiba, K., Savile, C., Fujii, R., Yu., J.-G., Gerassenkov, T., Kazlauskas, R.J., Bornscheuer, U.T. (2009), Converting an esterase into an epoxide hydrolase, Angew. Chem. Int. Ed. 48, 3584-3587, online.
Bartsch, S., Kourist, R., Bornscheuer, U.T. (2008), Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase, Angew. Chem. Int. Ed., 47, 1508-1511.
Höhne, M., Kühl, S., Robins, K., Bornscheuer, U.T. (2008), Efficient asymmetric synthesis of chiral amines by combining transaminase and pyruvate decarboxylase, ChemBioChem, 9, 363-365.
Hummel, A., Brüsehaber, E., Böttcher, D., Doderer, K., Trauthwein, H., Bornscheuer, U.T. (2007), Isoenzymes of pig liver esterase reveal striking differences in enantioselectivities, Angew. Chem. Int. Ed. 46, 8492-8494.
Kirschner, A., Bornscheuer, U.T. (2006), Kinetic resolution of 4-hydroxy-2-ketones by a Baeyer-Villiger monooxygenase, Angew. Chem. Int. Ed. 45, 7004-7006.
[27] Bornscheuer, U.T. (2010) Biocatalytic processes. In: Catalysis: From Molecular to Reactor Design, Beller, M., Renken, A., van Santen, R.A. (Eds.), Wiley-VCH, Weinheim, submitted.
[26] Bornscheuer, U.T. (2010) Biocatalysis. In: Catalysis: From Molecular to Reactor Design, Beller, M., Renken, A., van Santen, R.A. (Eds.), Wiley-VCH, Weinheim, submitted.
[25] Bornscheuer, U.T. (2010), Lipases, applications in synthesis of chiral compounds and in organic solvents, Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation and Cell Technology (Flickinger, M.C. Ed.)., John Wiley & Sons, Hoboken, accepted.
[24] Rehdorf, J., Bornscheuer, U.T. (2010), Monooxygenases, Baeyer-Villiger oxidations in organic synthesis, Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation and Cell Technology (Flickinger, M.C. Ed.)., John Wiley & Sons, Hoboken, accepted.
[23] Bornscheuer, U.T. (2010), Evolving enzymes for biocatalysis. In: Microbiology of Hydrocarbons, Oils, Lipids and Derived Compounds, Timmis, K. (Ed.), Springer, Berlin, in press.
[22] Böttcher, D., Schmidt, M., Bornscheuer, U.T. (2010), Screens for active and stereoselective hydrolytic enzymes. In: Molecular Methods in Metagenomics, Streit, W., Daniel, R., (Eds.), Springer, Berlin, accepted.
[21] Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2010), Directed evolution of industrial biocatalysts. In: Industrial Biotechnology, Soetaert, W., Vandamme, E. (Eds.), Wiley-VCH, Weinheim, pp. 155-187.
[20] Kirschner, A., Bornscheuer, U.T. (2009), Baeyer-Villiger monooxygenases in organic synthesis. In: Green Chemistry, Vol. 3: Biocatalysis, Anastas, P.T. (Ed.), Wiley-VCH, Weinheim, pp. 115-149.
[19] Böttcher, D., Schmidt, M., Bornscheuer, U.T. (2009), Alteration of substrate specificity and stereoselectivity of lipases and esterases. In: Protein Engineering, Bornscheuer, U.T., Lutz, S. (Eds.), Wiley-VCH, Weinheim, pp. 753-775.
[18] Kirschner, A., Bornscheuer, U.T. (2009), Enantioselective kinetic resolution of racemic 3-phenylbutan-2-one using a Baeyer-Villiger monooxygenase. In: Practical Methods in Biocatalysis and Biotransformations, Roberts, S.M., Whittall, J. (Eds.), Wiley-VCH, Weinheim, pp. 337-340.
[17] Hidalgo, A., Bornscheuer, U.T. (2006), Directed evolution of lipases and esterases for organic synthesis. In: Biocatalysis in the Pharmaceutical and Biotechnological Industries (Ed. R. Patel), Marcel Dekker, New York, pp. 159-179.
[16] Bornscheuer, U.T., Henke, E., Pleiss, J. (2005), Enzymatic Methods. In: Quaternary Stereocenters (Ed. Christoffers, J., Baro, A.), Wiley-VCH, Weinheim, pp. 315-327.
[15] Bornscheuer, U.T. (2005), Trends and Challenges in Enzyme Technology, Adv. Biochem. Eng. / Biotechnol., 100, 181-203.
[14] Bornscheuer, U.T. (2003), Assay systems for screening or selection of biocatalysts. In: Enzyme functionality: Design, engineering and screening (Svendsen, A, ed.) Marcel Dekker, New York, pp. 475-492.
[13] Bornscheuer, U.T., Adamczak, M., Soumanou, M.M. (2002) Lipase-catalyzed synthesis of modified lipids. In: Lipids as constituents of functional foods (Gunstone, FD, ed.) PJ Barnes & Associates, Bridgwater, pp. 149-182.
[12] Bornscheuer, U.T. (2002), Evolutionary Generation of Enzymes with Novel Substrate Specificities, in: Directed Evolution of Proteins; (Brakmann, S., Johnsson, K.; Ed.), Weinheim: Wiley-VCH, pp. 329-341.
[11] Yan, Y., Bornscheuer,U.T., Schmid, R.D. (2001), Application of crystallization technique for the lipase-catalyzed solid phase synthesisof sugar fatty acid monoesters; (Wildlak, N., Hartel, R., Narine, S.; Ed.),Champaign: AOCS Press, Vol. 15, 168-175.
[10] Schwaneberg, U., Bornscheuer, U.T. (2000), Fatty acid hydroxylations using P450 monooxygenases, in: Enzymes in Lipid Modification (Bornscheuer, U.T., Ed), Weinheim: Wiley-VCH, pp.394-414.
[9] Bornscheuer,U.T. (2000), Strategies for Improving the Lipase-Catalysed Preparation of Chiral Compounds, in: Methods and Tools in Biosciences and Medicine,Methods in Non-aqueous Enzymology (Gupta, M.N.; Ed.), Basel: Birkhäuser,pp. 90-109.
[8] Bornscheuer,U.T. (2000), Industrial Biotransformations, in: Biotechnology-Series(Rehm,H.J., Reed, G., Pühler, A. Stadler, P.J.W.; Eds.), Vol.8b, Weinheim: Wiley-VCH, pp. 277-294.
[7] Bornscheuer, U.T. (1999), ~250 keywords, in: Dictionary of Biotechnology and Gentechnology (in german language) (Deckwer, W.D., Pühler, A., Schmid, R.D.; Eds.). Stuttgart: Thieme, ISBN: 3137364027
[6] Bornscheuer, U.T., Pleiss, J., Schmidt-Dannert, C., Schmid, R.D. (1999), Lipases from Rhizopus oryzae: genetics, structure and applications, in.: Protein Engineeringin Industrial Biotechnology (Alberghina, L.; Eds.), 115-134. London: Harwood.
[5] Bornscheuer, U.T. (1999), Extending the applicability of lipases and esterases for organic synthesis, in.: Bioorganic Chemistry - Highlights and New Aspects (Diederichsen, U., Lindhorst, T.K., Wessjohann, L. , Westermann, B.; Eds.) (1999), Weinheim: Wiley-VCH, ISBN 3-527-29665-4, pp. 127-131.
[4] Bornscheuer, U.T. (1999), Recent advances in the lipase-catalyzed biotransformation of fats and oils, in.: Recent Research Developments in Oil Chemistry,Vol. 3,93-106. Trivandrum: Transworld Research Network.
[3] Bornscheuer, U., Padmanabhan, P., Scheper, T. (1999), Emulsion immobilized enzymes, in: Microspheres, microcapsules & liposomes (Arshady, R.; Ed.) 541-558. London: Citus Books.
[2] Kazlauskas, R.J., Bornscheuer, U.T. (1998), Biotransformations with Lipases, in.: Biotechnology-Series (Rehm, H.J., Reed, G., Pühler, A., Stadler, P.J.W., Kelly, D.R.; Eds.), Vol.8a, 37-191. Weinheim: Wiley-VCH.
[1] Soumanou, M.M., Schmid, U., Bornscheuer, U.T., Schmid, R.D. (1997), Lipasekatalysierte Synthesevon strukturierten Triglyceriden, in: Biokonversion nachwachsender Rohstoffe ,Vol.10, 184-190. Münster: Landwirtschaftsverlag.
Book
reviews
[7] Dynamic Stereochemistry of Chiral Compounds.
Principles and Applications, by C. Wolf, RSC Publishing, Cambridge, reviewed
in Angew. Chem. Int. Ed. (2008), 47, 5282; Angew. Chem. (2008), 120, 5362.
[6] Biocatalysis, by A. Bommarius, B.R. Riebel, Wiley-VCH, Weinheim, ISBN 3-527-30344-8, reviewed in Angew. Chem. Int. Ed. Engl. (2004), 43, 6584-6585; Angew. Chem. (2004), 116, 6746-6747.
[5] Taschenatlas der Biotechnologie und Gentechnik, by R.D. Schmid, Wiley-VCH, Weinheim, ISBN 3-527-30865-2, reviewed in Angew. Chem. (2002), 114, 3435.
[4] Enzymes: Biochemistry, Biotechnology, Clinical Chemistry. By T. Palmer. Horwood, Chichester, UK, ISBN 1-898563.78.0, reviewed in Angew. Chem. (2001), 113, 3358; .Angew. Chem. Int. Ed. Engl. (2001), 40, 3258-3259.
[3] Aqueous Two-Phase Systems. Methods and Protocols. Edited by R. Hatti-Kaul, Humana Press, Totawa, ISBN 0-896-03541-7, reviewed in Angew. Chem. (2000), 112, 4556-4557; .Angew. Chem. Int. Ed. Engl. (2000), 39, 4384-4385.
[2] Synthesis in Lipid Chemistry. Edited by J.H.P. Tyman, Royal Society of Chemistry, Cambridge, UK, ISBN 0-85404-716-6, reviewed in Fett/Lipid (1998), 100, 27.
[1] Lipoxygenase and Lipoxygenase Pathway Enzymes. Edited by G. J. Piazza, AOCS Press, Champaign, IL, USA, ISBN 0-935315-74-8, reviewed in Fett/Lipid (1997), 99, 152.
[9] Daum, G., Bornscheuer, U.T., Amoneit, F. (2010), Meeting report of the 7th Euro Fed Lipid Congress, Graz, Austria, 2009. Eur. J. Lipid Sci. Technol., 112, 275-287, link.
[8] Bornscheuer, U.T. (2010), Looking back into the future, Eur. J. Lipid Sci. Technol., 112, 153-154, link.
[7] Bornscheuer, U.T. (2009) Combined success for efficient catalysis, ChemCatChem 1, 5, link.
[6] Bornscheuer, U.T. (2009) Author profile, Angew. Chem. Int. Ed. 48, 5236, link; Angew. Chem.121, 5338, link.
[5] Kolisis, F., Bornscheuer, U.T., Amoneit, F. (2009), Report of the 6th Euro Fed Lipid Congress, Athens, Greece, 2008. Eur. J. Lipid Sci. Technol., 111, 303-309.
[4] Bornscheuer, U.T. (2009), Meeting report: Protein Design and Evolution for Biocatalysis. Biotechnol. J., 4, 444-446, link.
[3] Bornscheuer, U.T. (2008), Taking over a safe sailing boat heading to new discoveries, Eur. J. Lipid Sci. Technol., 110, 1, link.
[2] Bornscheuer, U.T. (2007), Editorial: Protein design and evolution for biocatalysis (special issue), Biotech. J., 2, 155, link.
[1] The
Biocatalysis and Biodegradation Database, reviewed in Angew. Chem. (2001),
113, 3817 (pdf); Angew.
Chem. Int. Ed. Engl. (2000), 40, 3703 (pdf).
[27] Bornscheuer, U.T., Hummel, A., Böttcher, D., Brüsehaber, E., Doderer, K., Trauthwein, H. (2008), New variants of pig liver esterase gamma for use in stereospecific hydrolysis. Intern. Patent PCT WO 2008116745; German Patent DE 102007014742.
[26] Robins, K., Bornscheuer, U., Höhne, M. (2008) Process for preparation of optically active n-protected 3 -aminopyrrolidine or optically active n-protected 3-aminopiperidine and the corresponding ketones by optical resolution of the racemic amine mixtures employing a bacterial omega-transaminase. Intern. Patent PCT WO 2008028654.
[25] Bornscheuer, U.T., Kirschner, A. (2008), Enantioselective representation of aliphatic acyclic esters and ketones, Intern. Patent PCT WO 2008022627.
[24] Liebeton, K., Eck,. J., Bornscheuer, U.T., Böttcher, D., Langer, P., Bellur, E. (2007), A novel group of esterases for the enantioselective production of fine and specialty chemicals, Intern. Patent PCT WO 2007128496.
[23] Robins, K., Bornscheuer, U., Höhne, M. (2007), Process for the preparation of optically active chiral amines. European Patent EP 1818411.
[22] Jackisch, B.O., Simmler-Hübenthal, H., Zschau, W., Bornscheuer, U.T., Durban, M., Riemer, C. (2007), Use of a thermostable phospholipase in the degumming of an oil or fat, and a method for obtaining a thermostable phospholipase, European Patent EP 1788080.
[21] Bornscheuer, U.T., Schmidt, M., Kähler, M., Rieks, A. (2006), New esterases and their use for processes for kinetic resolution of butinolesters, Intern. Patent PCT WO 07/042444.
[20] Bornscheuer, U.T., Grüning, B., Hills, G., Möbius, P., Thum, O., Weitemeyer, C. (2005), Process for the enzymatic synthesis of ethers. German Patent DE102005043669, US Patent 2007059789.
[19] Bornscheuer, U.T., Weiner, D.P., Hitchman, T., Lyon, J., Wongsakul, S. (2005), Hydrolases, nucleic acids encoding them and mehods for making and using them. European Patent EP 1601332; International Patent PCT WO 05/032496.
[18] Bornscheuer, U.T., Musidlowska-Persson, A., Trauthwein, H. (2004), Artificial esterases. German Patent DE 10258327.
[17] Bornscheuer, U.T., Konarzycka, M., Hills, G. (2003). Assay for synthesis activity of hydrolases in nonaqueous media using enzymic transesterification reaction releasing a carbonyl compd. European Patent EP 1354960.
[16] Bornscheuer, U.T., Baumann, M. (2002), Detection method for identifying hydrolases. German Patent DE 101 24 799; European Patent EP 1395673; International Patent PCT WO 02/94949.
[15] Short, J., Mathur, E., Baumann, M., Bornscheuer, U. (2002), Hydrolase enzymes and their use in kinetic resolution. International Patent WO 02/057411.
[14] Tscherry, B., Bornscheuer, U., Musidlowska, A., Werlen, J. Zimmermann, T. (2002), Manufacture of L-carnitine from betaine esters with microorganisms bearing a betaine ester hydrolase. European Patent EP 1370663; International Patent PCT WO 02/061094.
[13] Riermeier, T., Bornscheuer, U., Altenbuchner, J., Hildebrandt, P. (2002), An alcohol dehydrogenase from Pseudomonas fluorescens for use in the manufacture of alcohols from ketones. German Patent DE 10112401; European Patent EP 1241263.
[12] Bornscheuer, U., Musidlowska, A., Schmidt-Dannert, C., Lange, S. (2002), Rekombinante Schweineleberesterase, deren Verwendung sowie ein Verfahren zu deren Herstellung. German Patent DE 10061864; European Patent EP 1343902; International Patent PCT WO 02/048322.
[11] Wessjohann L.A., Scheid G., Bornscheuer U., Henke E., Kuit W., Orru R. (2002), Epothilone synthesis components III and IV: Asymmetrically substituted acyloins and acyloin derivatives, method for the production thereof and method for the production of Epothilone B, D and Epothilone derivatives. German Patent DE 10134172 ; European Patent EP 1358144; US Patent US 6,867,333; International Patent PCT WO 02/32844.
[10] Bornscheuer, U.T., Meyer, H.H., Altenbuchner, J., (2002), Alteration of substrate specificity of enzymes, German Patent DE 19743683; European Patent EP 0909821; US Patent US 6,365,398.
[9] Gatfield, I., Hilmer, J.M., Bornscheuer, U., Schmid, R.D., Vorlova, S., (2002), Method for preparing D- and L-menthol. German Patent DE 10100913; European Patent EP 1223223; US Patent US 6,706,500.
[8] Brocca, S., Bornscheuer, U., Pleiss, J., Schmid, R.D., Schmid, U., Schmitt, J. Modified lypolytic enzymes and their use. European Patent EP 1130100.
[7] Bornscheuer, U., Henke, E., Yang, H. (2002), Verfahren zur Herstellung von stereoisomeren Carbonsäureestern, German Patent DE 19908074; European Patent EP 1031629; US Patent US 6,365,398.
[6] Yang,H., Henke, E., Bornscheuer, U.T. (2000), Verfahren zur Racematspaltung von Arylalkylcarbonsäureestern. German Patent DE 19844876; European Patent EP 0997534; US Patent US 6,201,147.
[5] Bornscheuer, U.T, Schmid, R.D., Scheib, H., Otto, R.T., Syldatk, C. (2000), Method for the production of uronic acid esters in a lipase-catalyzed process. German Patent DE 19839219.
[4] Bornscheuer, U., Schmid, R.D., Syldatk, C., Youchun, Y., Otto, R. (2000), Verfahren zur selektiven Veresterung von Polyolen. German Patent DE 19924221.
[3] Otto, R., Syldatk, C., Cao, L., Bornscheuer, U., Schmid, R.D. (1999), Verfahren zur selektiven Veresterung von Polyolen. German Patent DE 19753789; European Patent EP 1175500.
[2] Bornscheuer, U.T., Soumanou, M.M., Schmid, R.D., Schmid, U. (1998), Preparation of symmetrical triglycerides ABA, European Patent EP 0882797.
[1] Minning, S., Bornscheuer, U., Schmid, R.D. (1998), Azo dye for detection of percarboxylic acids, German Patent DE 19651797.
Publication and Patent List
(for a listing as pdf-file click here)
[219] Kuipers, R.K., Joosten, H.J., van Berkel, W.J.H., Leferink, N.G.H., Rooijen, E., Ittmann, E., van Zimmeren, F., Jochens, H., Bornscheuer, U., Vriend, G., Martins dos Santos, V.A.P., Schaap, P.J. (2010), 3DM: systematic analysis of heterogeneous super-family data to discover protein functionalities, Proteins, accepted.
[218] Rehdorf, J., Mihovilovic, M.D., Bornscheuer, U.T. (2010), Exploiting regioselectivity of Baeyer-Villiger-monooxygenases – a route to the formation of ß-amino acids and ß-amino alcohols, Angew. Chem. Int. Ed., accepted.
[217] Gall, M., Kourist, R., Schmidt, M., Bornscheuer, U.T. (2010), The role of the GGG(A)X-motif on enantioselectivity of pig liver esterase towards tertiary alcohols, Biocat. Biotransform, accepted.
[216] Nguyen, G.S., Kourist, R., Paravidino, M., Hummel, A., Rehdorf, J., Orru, R.V.A., Hanefeld, U., Bornscheuer, U.T. (2010), An enzymatic toolbox for the kinetic resolution of 2-(pyridinyl)but-3-yn-2-ols and tertiary cyanohydrins, Eur. J. Org. Chem., accepted.
[215] Wittrup-Larsen, M., Zielinska, D.F., Martinelle, M., Hidalgo, A., Jensen, L.J., Bornscheuer, U.T., Hult, K. (2010), Suppression of water as a nucleophile in Candida antarctica lipase B catalysis, ChemBioChem, link.
[214] Schätzle, S., Höhne, M., Robins, K., Bornscheuer U.T. (2010), A conductometric method for the rapid characterization of the substrate specificity of amine-transaminases, Anal. Chem., 82, 2082-2086, link.
[213] Böttcher, D., Bornscheuer, U.T. (2010) Protein engineering of microbial enzymes, Curr. Opin. Microbiol., link.
[212] Brüsehaber, E., Schwiebs, A., Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2010), Production of pig liver esterase in batch fermentation of E. coli Origami, Appl. Microb. Biotechnol., link.
[211] Rzeznicka, K., Schätzle, S., Böttcher, D., Klein, J., Bornscheuer, U.T. (2010), Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterization, Appl. Microbiol. Biotechnol., 85, 1417-1425, link.
[210] Wardenga, R., Lindner, H.A., Hollmann, F., Thum, O., Bornscheuer, U.T. (2010) Increasing the synthesis/ hydrolysis ratio of aminoacylase-1 by site-directed mutagenesis, Biochimie, 92, 102-109, link.
[209] Kourist, R., Brundiek, H., Bornscheuer, U.T. (2010), Protein engineering and discovery of lipases, Eur. J. Lipid Sci. Technol., 112, 64-74, link.
[208] Fernandez-Alvaro, E., Kourist, R., Winter, J., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Jäger, K.E., Streit, W. Bornscheuer, U.T. (2009), Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases, Microb. Biotechnol., 3, 59-64, link.
[207] Bornscheuer, U.T. (2009) Modern tools in the discovery and design of biocatalysts, Enzyme Engineering News Japan, 62, 5-14.
[206] Brüsehaber, E., Böttcher, D., Bornscheuer, U.T. (2009), Insights into the physiological role of pig liver esterase: Isoenzymes show differences in the demethylation of prenylated proteins, Bioorg. Med. Chem., 17, 7878-7883, link.
[205] Schließmann, A., Hidalgo, A., Berenguer, J., Bornscheuer, U.T. (2009) Increased enantioselectivity by engineering bottleneck mutants in an esterase from Pseudomonas fluorescens, ChemBioChem, 10, 2920-2923, link.
[204] Schmidt, M., Böttcher, D., Bornscheuer, U.T. (2009), Protein engineering of carboxyl esterases by rational design and directed evolution, Protein Peptide Lett., 16, 1162-1171.
[203] Thodi, K., Barbayianni, E., Fotakopoulou, I., Bornscheuer, U.T., Constantinou-Kokotou, V., Moutevelis-Minakakis, P., Kokotos, G. (2009), Study of the removal of allyl esters by Candida antarctica lipase B (CAL-B) and pig liver esterase (PLE), J. Mol. Catal. B: Enzymatic, 61, 241-246, link.
[202] Schätzle, S., Höhne, M., Redestad, E., Robins, K., Bornscheuer U.T. (2009), A rapid and sensitive kinetic assay for characterization of omega-transaminases, Anal. Chem, 81, 8244-8248, link.
[201] Barbayianni, E., Kokotos, C.G., Bartsch, S., Drakou, C., Bornscheuer, U.T., Kokotos, G. (2009), Bacillus subtilis esterase (BS2) and its double mutant have different selectivity in the removal of carboxyl protecting groups, Adv. Synth. Catal., 351, 2325-2332, link.
[200] Adamczak, M., Bednarski, W., Bornscheuer, U.T. (2009), The application of biotechnological methods for synthesis of biodiesel, Eur. J. Lipid Sci. Technol., 111, 808-813 link.
[199] Kazlauskas, R.J., Bornscheuer U.T. (2009), Finding better protein engineering strategies, Nat. Chem. Biol., 5, 526-529, link.
[198] Royter, M., Schmidt, M., Elend, C., Höbenreich, H., Schäfer, T., Bornscheuer, U.T., Antranikian, G. (2009), Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis, Extremophiles, 13, 769-783, link.
[197] Höhne, M., Bornscheuer, U.T. (2009), Biocatalytic routes to optically active amines, ChemCatChem, 1, 42-51, link.
[196] Rehdorf, J., Lengar, A., Bornscheuer, U.T., Mihovilovic, M.D. (2009), Kinetic resolution of arylaliphatic acyclic ß-hydroxyketones by recombinant whole-cell Baeyer-Villiger monooxygenases – formation of enantiocomplementary regioisomeric esters, Bioorg. Med. Chem. Lett., 19, 3739-3743, link.
[195] Wigger, M., Holt, J., Kourist, R., Bartsch, S., Arends, I.W.C.E., Minnaard, A.J., Bornscheuer, U.T., Hanefeld, U. (2009), Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols, J. Mol. Catal. B: Enzymatic, 60, 82-86, link.
[194] Kourist, R., Höhne, M., Bornscheuer, U.T. (2009), Protein Design: Im Spannungsfeld zwischen gerichteter Evolution und rationalem Design, Chemie in unserer Zeit, 43, 132-142, link.
[193] Bartsch, S., Bornscheuer, U.T. (2009), A single residue influences the reaction mechanism of ammonia lyases and mutases, Angew. Chem. Int. Ed., 48, 3362-3365, link; Einfluss einer einzelnen Aminosäure auf den Reaktionsmechanismus von Ammonium-Lyasen und -Mutasen, Angew. Chem., 121, 3412-3415, link.
[192] Jochens, H., Stiba, K., Savile, C., Fujii, R., Yu, J.-G., Gerassenkov, T., Kazlauskas, R.J., Bornscheuer, U.T. (2009), Converting an esterase into an epoxide hydrolase, Angew. Chem. Int. Ed., 48, 3532-3535, link; Umwandlung einer Esterase in eine Epoxidhydrolase, Angew. Chem., 121, 3584-3587, link.
[191] Rehdorf, J., Zimmer, C., Bornscheuer, U.T. (2009), Cloning, expression and biocatalytic investigation of 4-hydroxyacetophenone monooxygenase from Pseudomonas putida JD1, Appl. Environm. Microbiol., 75, 3106-3114, link
[190] Rachinskiy, K., Schultz, H, Boy, M., Bornscheuer, U.T., Büchs, J. (2009), Enzyme test bench: a high-throughput enzyme characterization technique including the long term stability, Biotechnol. Bioeng. 103, 302-322, link.
[189] Krause, P., Hilterhaus, L., Fieg, G., Liese, A., Bornscheuer, U.T. (2009), Chemically and enzymatically catalyzed synthesis of C6-C10 alkyl benzoates, Eur. J. Lipid Sci. Technol., 111, 194-201, link.
[188] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2009), Improving ascorbyl oleate synthesis by application of ionic liquids and water activity control by salt hydrates, Proc. Biochemistry, 44, 257-261, link.
[187] Wardenga, R., Hollmann, F., Thum, O., Bornscheuer, U.T. (2008) Functional expression of porcine aminoacylase 1 in E. coli using a codon optimized synthetic gene and molecular chaperones, Appl. Microbiol. Biotechnol., 81, 721-729, link.
[186] Heinze, B., Hoven, N., O'Connell, T., Maurer, K.H., Bartsch, S., Bornscheuer, U.T. (2008) Recovery of choline oxidase activity by in vitro recombination of individual segments, Appl. Microbiol. Biotechnol., 81, 275-282, link.
[185] Kirschner, A., Bornscheuer, U.T. (2008), Directed evolution of a Baeyer-Villiger monooxygenase to enhance enantioselectivity, Appl. Microbiol. Biotechnol., 81, 465-472, link.
[184] Bornscheuer, U.T. (2008), Alteration of lipase properties by protein engineering methods, Oléagineux Corps Gras Lipides, 15, 184-188.
[183] Wittrup-Larsen, M., Bornscheuer, U.T., Hult, K. (2008), Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems, Prot. Expr. Purif., 62, 90-97, link.
[182] Kourist, R., Nguyen, G.S., Strübing, S., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Bornscheuer, U.T. (2008), Hydrolase-catalyzed stereoselective preparation of protected a,a-dialkyl-a-hydroxycarboxylic acids, Tetrahedron: Asymmetry, 19, 1839-1843, link.
[181] Hidalgo, A., Schliessmann, A., Molina, R. Hermoso, J., Bornscheuer, U.T. (2008), An one-pot, simple methodology for cassette randomization and recombination for focused directed evolution (OSCARR), Prot. Des. Eng. Sel., 21, 567-576, link.
[180] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2008), Properties and biotechnological methods to produce lipids containing conjugated linoleic acid (CLA), Eur. J. Lipid Sci. Technol., 110, 491-502, link.
[179] Höhne, M., Robins, K., Bornscheuer, U.T. (2008), A protection strategy substantially enhances rate and enantioselectivity in transaminase-catalyzed kinetic resolutions, Adv. Synth. Catal., 350, 807-812, link.
[178] Brüsehaber, E., Böttcher, D., Liebeton, E., Eck, J., Naumer, C., Bornscheuer, U.T. (2008), Asymmetric synthesis of cis-3,5-diacetoxycyclopent-1-ene using metagenome derived hydrolases, Tetrahedron: Asymmetry, 19, 730-732, link.
[177] Kourist, R., Dominguez de Maria, P., Bornscheuer, U.T. (2008), Enzymatic synthesis of optically active tertiary alcohols: Expanding the biocatalytic toolbox, ChemBioChem, 9, 491-498, link.
[176] Vahl, K., Kahlert, H., Böttcher, D., Wardenga, R., Komorsky-Lovric, S., Bornscheuer, U., Scholz, F. (2008), A potential high-throughput method for the determination of lipase activity by potentiometric FIA titrations, Anal. Chem. Acta, 610, 44-49, link.
[175] Bartsch, S., Kourist, R., Bornscheuer, U.T. (2008), Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase, Angew. Chem. Int. Ed., 47, 1508-1511 link; Vollständige Umkehrung der Enantioselektivität gegen acetylierte tertiäre Alkohole durch eine Doppelmutation in einer Esterase aus Bacillus subtilis, Angew. Chem., 120, 1531-1534, link.
[174] Höhne, M., Kühl, S., Robins, K., Bornscheuer, U.T. (2008), Efficient asymmetric synthesis of chiral amines by combining transaminase and pyruvate decarboxylase, ChemBioChem, 9, 363-365, link.
[173] Völker, A., Kirschner, A., Bornscheuer, U.T., Altenbuchner, J. (2008), Functional expression, purification and characterization of the recombinant Baeyer-Villiger monooxygenase MekA from Pseudomonas veronii MEK700, Appl. Microbiol. Biotechnol., 77, 1251-1260, link.
[172] Bertram, M. Hildebrandt, P., Weiner, D.W., Patel, J. S., Bartnek, F., Hitchman, T., Bornscheuer, U.T. (2008), Characterization of lipases and esterases from metagenomes for lipid modification, J. Am. Oil Chem. Soc., 85, 47-53, link.
[171] Kourist, R., Bartsch, S., Fransson, L., Hult, K., Bornscheuer, U.T. (2008), Understanding promiscuous amidase activity of an esterase from Bacillus subtilis, ChemBioChem, 9, 67-69, link.
[170] DiLorenzo, M., Hidalgo, A., Molina, R., Hermoso, J.A., Pirozzi, D., Bornscheuer, U.T. (2007), Enhancement of the stability of a prolipase from Rhizopus oryzae towards aldehydes by saturation mutagenesis, Appl. Environm. Microbiol. 73, 7291-7299, link.
[169] Hummel, A., Brüsehaber, E., Böttcher, D., Doderer, K., Trauthwein, H., Bornscheuer, U.T. (2007), Isoenzymes of pig liver esterase reveal striking differences in enantioselectivities, Angew. Chem. Int. Ed. 46, 8492-8494, link ; Hummel, A., Brüsehaber, E., Böttcher, D., Doderer, K., Trauthwein, H., Bornscheuer, U.T. (2007), Isoenzyme der Schweineleberesterase zeigen bemerkenswerte Unterschiede in ihrer Enantioselektivität, Angew. Chem. 119, 8644-8646, link.
[168] Gruber, C.C., Nestl, B.M., Gross., J., Hildebrandt, P., Bornscheuer, U.T., Faber, K., Kroutil, W. (2007), Emulation of racemase activity by employing a pair of stereocomplementary biocatalysts, Chem. Eur. J., 13, 8271-8276, link.
[167] Kourist, R., Krishna, S.H., Patel, J.S., Bartnek, F., Weiner, D.W., Hitchman, T., Bornscheuer, U.T. (2007), Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols, Org. Biomol. Chem., 5, 3310-3313, link.
[166] Brüsehaber, E., Böttcher, D., Musidlowska-Persson, A., Albrecht, D., Hecker, M., Doderer, K., Bornscheuer, U.T. (2007), Identification of pig liver esterase variants by tandem mass spectroscopy analysis and their characterization, Appl. Microbiol. Biotechnol., 76, 853-859, link.
[165] Rehdorf, J., Kirschner, A., Bornscheuer, U.T. (2007), Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440, Biotechnol. Lett., 29, 1393-1398, link.
[164] Kourist, R., Bartsch, S., Bornscheuer, U.T., (2007), Highly enantioselective synthesis of arylaliphatic tertiary alcohols using mutants of an esterase from Bacillus subtilis, Adv. Synth. Catal., 349, 1393-1398, link.
[163] Durban, M., Bornscheuer, U.T. (2007), An improved assay for the determination of phospholipase C activity, Eur. J. Lipid Sci. Technol., 109, 469-473, link.
[162] Geitner, K., Kirschner, A., Rehdorf, J., Schmidt, M., Mihovilovic, M.D., Bornscheuer, U.T. (2007), Enantioselective kinetic resolution of 3-phenyl-2-ketones using Baeyer-Villiger monooxygenases, Tetrahedron: Asymmetry, 18, 892-895, link.
[161] Kirschner, A., Altenbuchner, J., Bornscheuer, U.T. (2007), Design of a secondary alcohol degradation pathway from Pseudomonas fluorescens DSM50106 in an engineered E. coli, Appl. Microbiol. Biotechnol., 75, 1095-1101, link.
[160] Heinze, B., Kourist, R., Fransson, L., Hult, K., Bornscheuer, U.T. (2007), Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis, Prot. Eng. Des. Sel., 20, 125-131, link.
[159] Bertram, M., C. Manschot-Lawrence, E. Flöter, Bornscheuer, U.T. (2007), A microtiter plate-based assay method to determine fat quality, Eur. J. Lipid Sci. Technol., 109, 180-185, link.
[158] Fotakopoulou, I., Barbayianni, E., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2007), Enzymatic removal of carboxyl protecting groups. Part III: Fast removal of the allyl and chloroethyl esters by Bacillus subtilis esterase (BS2), J. Org. Chem., 72, 782-786, link.
[157] Durban, M., Silbersack, J., Schweder, T., Schauer, F., Bornscheuer, U.T. (2007), High level expression of a recombinant phospholipase C from Bacillus cereus in B. subtilis, Appl. Microbiol. Biotechnol., 74, 634-639, link.
[156] Schmidt, M., Henke, E., Heinze, B., Kourist, R., Hidalgo, A., Bornscheuer, U.T. (2007), A versatile esterase from Bacillus subtilis: Cloning, expression, characterization, and its application in biocatalysis, Biotech. J., 2, 249-253, link.
[155] Böttcher, D., Brüsehaber, E., Doderer. K., Bornscheuer, U.T. (2007), Functional expression of the gamma-isoenzyme of pig liver carboxyl esterase in Escherichia coli, Appl. Microbiol. Biotechnol., 73, 1282-1289, link.
[154] Kirschner, A., Altenbuchner, J., Bornscheuer, U.T. (2007), Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas fluorescens DSM50106 in E.coli, Appl. Microbiol. Biotechnol., 73, 1065-1072, link.
[153] Böttcher, D., Bornscheuer, U.T. (2006), High-throughput screening of activity and enantioselectivity of esterases, Nature Protocols, 1, 2340-2343, online, link.
[152] Kirschner, A., Bornscheuer, U.T. (2006), Katalytische kinetische Racematspaltung von 4-Hydroxy-2-Ketonen durch eine Baeyer-Villiger Monooxygenase, Angew. Chem., 118, 7161-7163 (german version), link; Kirschner, A., Bornscheuer, U.T. (2006), Kinetic resolution of 4-hydroxy-2-ketones by a Baeyer-Villiger monooxygenase, Angew. Chem. Int. Ed. 45, 7004-7006 (english version), link.
[151] Bellur, E., Freifeld, I., Holtz, E., Böttcher, D., Bornscheuer, U.T., Langer, P. (2006), Synthesis of (tetrahydrofuran-2-yl)acetates and (pyrrolidin-2-yl)acetates based on a 'cyclization / hydrogenation' strategy, Tetrahedron, 62, 7132-7139, link.
[150] Metzger, J.O., Bornscheuer, U.T. (2006), Lipids as renewable resources: Current state of chemical and biotechnological conversion and diversification, Appl. Microbiol. Biotechnol., 71, 13-22, link.
[149] Chaparro-Riggers, J.F., Breves, R., Maurer, K.H., Bornscheuer U.T. (2006), Modulation of infectivity in phage display as a tool to determine the substrate specificity of proteases, ChemBioChem., 7, 965-970, link.
[148] Bellur, E., Böttcher, D., Bornscheuer, U.T., Langer, P. (2006), Enantioselective synthesis of 2-alkylidenetetrahydrofurans based on a 'cyclization / enzymatic resolution' strategy, Tetrahedron: Asymmetry, 17, 892-899, link.
[147] Brandt, B., Hidalgo, A., Bornscheuer, U.T. (2006), Immobilization of enzymes in the microtiter plate scale, Biotech. J., 1, 582-587, link.
[146] Schmidt, M., Hasenpusch, D., Kähler, M., Kirchner, U., Wiggenhorn, K., Langel, W., Bornscheuer, U.T. (2006), Directed evolution of an esterase from Pseudomonas fluorescens yields a mutant with excellent enantioselectivity and activity for the kinetic resolution of a chiral building block, ChemBioChem., 7, 805-809, link.
[145] Ganske, F., Bornscheuer, U.T. (2006), Growth of Escherichia coli, Pichia pastoris and Bacillus cereus in the presence of the ionic liquids [BMIM][BF4] and [BMIM][PF6] and organic solvents, Biotechnol. Lett., 28, 465-469, link.
[144] Xu, J.-H., Zhou, R., Bornscheuer, U.T. (2005), Comparison of differently modified Pseudomonas cepacia lipases in enantioselective preparation of a chiral alcohol for agrochemical use, Biocat. Biotransform., 23, 415-422, link .
[143] Di Lorenzo, M., Hidalgo, A., Haas, M., Bornscheuer, U.T. (2005), Heterologous production of functional Rhizopus oryzae lipase forms in Eschericha coli, Appl. Environm. Microbiol., 71, 8974-8977, link.
[142] Sareen, R., Bornscheuer, U.T., Mishra, P., Cloning, functional expression and characterization of an alkaline, organic solvent tolerant protease from Bacillus licheniformis RSP-09-37, Biotechnol. Lett., 27, 1901-1907, link.
[141] Poessl, T.M., Kosjek, B., Ellmer, U., Gruber, C.C., Edegger, K., Faber, K. Hildebrandt, P., Bornscheuer, U.T., Kroutil, W., Stereo-complementary non-racemic halohydrins via biocatalytic hydrogen transfer-reduction of halo-ketones and one-pot cascade-reaction to enantiopure epoxide, Adv. Synth. Catal., 345, 1827-1834, link.
[140] Ödman, P., Wessjohann, L.A., Bornscheuer, U.T. (2005), Chemoenzymatic dynamic kinetic resolution of acyloins, J. Org. Chem., 70, 9551-9555, link.
[139] Barbayianni, E., Fotakopoulou, I., Schmidt, M., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2005), Enzymatic removal of carboxyl protecting groups. Cleavage of the benzyl- and methyl moieties, J. Org. Chem., 70, 8730-8733, link.
[138] Ganske, F., Bornscheuer, U.T. (2005), Optimization of lipase-catalyzed glucose fatty acid ester synthesis in a two-phase systems containing ionic liquids and t-BuOH, J. Mol. Catal. B: Enzym., 36, 40-42, link.
[137] Bornscheuer, U.T., Rodriguez Ordonez, G., Hidalgo, A., Gollin, A., Lyon, J., Hitchman, T.S., Weiner, D.P. (2005), Selectivity of lipases and esterases towards phenol esters, J. Mol. Catal. B: Enzymatic, 36, 8-13, link.
[136] Buchholz, K., Bornscheuer, U.T. (2005), Highlights in biocatalysis – Historical landmarks and current trends, Eng. Life Sci., 5, 309-323, link.
[135] Bornscheuer, U.T. (2005), Deep-sea mining for unique biocatalysts, Chem. Biol., 12, 859-860, link.
[134] Adamczak, M., Bornscheuer, U.T., Bednarski, W. (2005), Synthesis of ascorbyloleate by immobilized Candida antarctica lipases, Process Biochemistry, 40, 3177-3180, link.
[133] Bornscheuer U.T. (2005), White biotechnology for lipids, fats and oils, Eur. J. Lipid Sci. Technol., 107, 445-446, link.
[132] Chaparro-Riggers, J.F., Breves, R., Michels, A., Maurer, K.H., Bornscheuer U.T. (2005), A GFP-based assay for the determination of hydrolytic activity and substrate specificity of subtilisins under washing conditions, J. Mol. Catal. B: Enzymatic, 35, 74-77, link.
[131] Ganske, F., Bornscheuer, U.T. (2005), Lipase-catalyzed glucose fatty acid ester synthesis in ionic liquids, Org. Lett., 7, 3097-3098, link.
[130] Strübing, D., Kirschner, A., Neumann, H., Hübner, S., Klaus, S., Bornscheuer, U.T., Beller, M. (2005), Synthesis of enantiomerically pure cyclohex-2-en-1-ols: Development of novel multicomponent reactions, Chem. Eur. J., 11, 4210-4218, link.
[129] Schmidt, M., Bornscheuer, U.T. (2005), High-throughput assays for lipases and esterases, Biomolecular Eng., 22, 51-56, link.
[128] Robertson, D.E., Bornscheuer, U.T. (2005), Biocatalysis and biotransformation. New technologies, enzymes and challenges, Curr. Opin. Chem. Biol., 9, 164-165, link.
[127] Schmidt, M., Barbayianni, E., Fotakopoulou, I., Höhne, M., Constantinou-Kokotou, V., Bornscheuer, U.T., Kokotos, G. (2005), Enzymatic Removal of Carboxyl Protecting Groups. Part I: Cleavage of the tert-Butyl Moiety, J. Org. Chem., 70, 3737-3040, available online as ASAP article, link.
[126] Soumanou, M., Aleodjrodo, P., Bornscheuer, U.T. (2004), Activity and stability of immobilized lipases in lipase-catalyzed modification of peanut oil, Oleagineux, Corps Gras, Lipides, 11, 464-468.
[125] Sareen, R., Bornscheuer, U.T., Mishra, P. (2004), Synthesis of Kyotorphin precursor by an organic solvent–stable protease from Bacillus licheniformis RSP-09-37, J. Mol. Catal. B: Enzym., 32, 1-5, link.
[124] Bornscheuer, U.T. (2004), High-throughput-screening systems for hydrolases, Eng. Life. Sci., 4, 539-542, link.
[123] Bornscheuer, U.T., Kazlauskas, R.J. (2004), Catalytic promiscuity in biocatalysis: Using old enzymes to form new bonds and follow new pathways, Angew. Chem. Int. Ed. Engl., 42, 6032-6040, link; Untreue Enzyme in der Biokatalyse: Mit alten Enzymen zu neuen Bindungen und Synthesewegen, Angew. Chem., 116, 6156-6165, link.
[122] Wongsakul, S., Bornscheuer, U.T. Kittikun, H. (2004), Lipase-catalyzed acidolysis and Phospholipase D-catalyzed transphosphatidylation of phosphatidylcholine, Eur. J. Lipid Sci. Technol., 106, 665-670, link.
[121] Scheid, G., Ruijter, E., Konarzycka-Bessler, M., Bornscheuer, U.T., Wessjohann, L.A. (2004), Synthesis and resolution of a key building block for epothilones – a comparison of asymmetric synthesis, chemical, and enzymatic resolution, Tetrahedron: Asymmetry, 15, 2861-2869, link.
[120] Kirschner, A., Langer, P., Bornscheuer, U.T. (2004), Lipase-catalyzed highly enantioselective kinetic resolution of alpha-hydroxy butenolides, Tetrahedron: Asymmetry, 15, 2871-2874, link.
[119] Bornscheuer, U.T. (2004), Finding enzymatic gold on silver surfaces, Nat. Biotechnol., 22, 1098-1099, pdf, link .
[118] Sareen, R., Bornscheuer, U.T., Mishra, P. (2004), A microtiter plate assay for the determination of the synthetic activity of protease, Anal. Biochem., 333, 193-195, link.
[117] Schmidt, M., Baumann, M., Henke, E., Konarzycka-Bessler, M., Bornscheuer U.T. (2004), Directed evolution of lipases and esterases, Methods Enzymol., 388, 199-207.
[116] Wongsakul, S., Kittikun, A., Bornscheuer, U.T. (2004), Lipase-catalyzed synthesis of structured triglycerides from 1,3-diglycerides, J. Am. Oil Chem. Soc., 81, 151-155.
[115] Schönfeld, D.L., Bornscheuer, U.T. (2004), A polarimetric assay for the medium-throughput determination of amino acid racemase activity, Anal. Chem., 76, 1184-1188, link.
[114] Wessjohann, L.A., Scheid, G., Orru, R.V.A., Kuit, W., Ruijter, E., Henke, E., Bornscheuer, U.T. (2004), A new route to protected acyloins and their enzymatic resolution with lipases, Eur. J. Org. Chem., 1063-1074, link.
[113] Musidlowska-Persson, A., Bornscheuer, U.T. (2003), Recombinant porcine intestinal carboxyl esterase: Cloning from the pig liver esterase gene by site-directed mutagenesis, functional expression and characterization, Protein Eng., 16, 1139-1145, link.
[112] Soumanou, M.M., Bornscheuer, U.T. (2003), Lipase-catalyzed alcoholysis of vegetable oils, Eur. J. Lipid Sci. Technol., 105, 656-660, link.
[111] Durban, M., Bornscheuer, U.T. (2003), An assay system for the detection of Phospholipase C activity, Eur. J. Lipid Sci. Technol., 105, 633-637, link.
[110] Ganske, F., Meyer, H.H., Deutz, H., Bornscheuer, U.T. (2003), Enzyme-catalyzed hydrolysis of 18-methyl eicosanoic acid-cysteine thioester, Eur. J. Lipid Sci. Technol., 105, 627-632, link.
[109] Bornscheuer, U.T., Musidlowska-Persson, A., Böttcher, D., Liebeton, K., Lorenz, P., Eck, J., Zinke, H., Schleper, C., Langer, P., Trauthwein, H., Karau, A., Buchholz, S. (2003), Esterasen und Lipasen aus kultivierten und nicht-kultivierten Mikroorganismen, Transkript, 9 (10), 81-84.
[108] Kähler, M., Rieks, A., Kirchner, U., Wiggenhorn, K., Bornscheuer, U.T., Schmidt, M., Eisner, A., Petersen, W., Ellerbrock, F., Bollow, S. (2003), Biokatalytische Synthese enantiomerenreiner building blocks, Transkript, 9 (10), 78-80.
[107] Bornscheuer, U.T. (2003), Immobilizing enzymes: how to create more suitable biocatalysts, Angew. Chem. Int. Ed. Engl. 42, 3336-3337, link; Enzymimmobilisierung: ein Weg zu verbesserten Biokatalysatoren, Angew. Chem., 115, 3458-3459, link.
[106] Soumanou, M.M., Bornscheuer, U.T. (2003), Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil to Biodiesel using immobilized lipases, Enzyme Microb. Technol., 33, 97-103, link.
[105] Henke, E., Bornscheuer, U.T., Schmid, R.D., Pleiss, J. (2003), The molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases, Chem. Bio. Chem., 4, 485-493, link.
[104] Musidlowska-Persson, A., Bornscheuer,
U.T. (2003), Site-directed mutagenesis of recombinant pig liver esterase
yields mutants with altered enantioselectivity, Tetrahedron: Asymmetry, 14,
1341-1344, link.
[103] Horsman, G.P., Liu, A.F.M., Henke, E., Bornscheuer, U.T., Kazlauskas, R.J. (2003), Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase toward methyl 3-Bromo-2-methylpropanoate and ethyl 3-phenylbutyrate, Chem. Eur. J., 9, 1933-1939, link.
[102] Konarzycka-Bessler, M. Bornscheuer, U.T. (2003), A high-throughput-screening method for the determination of the synthetic activity of hydrolases, Angew. Chem. Int. Ed., 42, 1418-1420, link; Eine Hochdurchsatz-Screeningmethode zur Bestimmung der Syntheseaktivität von Hydrolasen, Angew. Chem., 115, 1449-1451, link.
[101] Persson, M.M., Bornscheuer, U.T. (2003), Increased stability of an esterase from Bacillus subtilis in ionic liquids as compared to organic solvents, J. Mol. Catal. B: Enzymatic, 22, 21-27, link.
[100] Wongsakul, S., Prasertsan, P., Bornscheuer, U.T., H-Kittikun, A. (2003), Synthesis of 2-monoglycerides by alcoholysis of palm oil and tuna oil using immobilized lipases, Eur. J. Lipid Sci. Technol., 105, 68–73, link.
[99] Henke, E., Bornscheuer, U.T. (2003), Fluorophoric assay for the high-throughput determination of amidase activity, Anal. Chem., 75, 255-260 (in press (DOI), link.
2002
[98] Hari Krishna, S., Persson,
M.M., Bornscheuer, U.T. (2002), Enantioselective transesterification of a
tertiary alcohol by Lipase A from Candida antarctica, Tetrahedron: Asymmetry,
13, 2693-2696.
[97] Bornscheuer, U.T. (2002), Methods to increase enantioselectivity of lipases and esterases, Curr. Opin. Biotechnol., 13, 543-547.
[96] Vorlová, Bornscheuer, U.T., Gatfield, I., Hilmer, J.M., Bertram, H.J., Schmid, R.D., (2002), Enantioselective hydrolysis of D,L-menthyl benzoate to L-menthol by recombinant Candida rugosa lipases, Adv. Synth. Catal., 344, 1152-1155, link.
[95] Henke, E., Bornscheuer, U.T., (2002), Esterases from Bacillus subtilis and Bacillus stearothermophilus share high sequence homology but differ substantially in their properties, Appl. Microbiol. Biotechnol., 60, 320-326, link.
[94] Musidlowska-Persson, A., Bornscheuer, U.T. (2002), Substrate specificity of the gamma-isoenzyme of recombinant pig liver esterase towards acetates of secondary alcohols, J. Mol. Catal. B: Enzymatic, 19-20C, 129-133.
[93] Bornscheuer, U.T., Bessler, C., Srinivas, R., Krishna, S.H. (2002), Optimizing lipases and related enzymes for efficient application, Trends Biotechnol., 20, 433-437.
[92] Henke, E., Pleiss, J., Bornscheuer, U.T. (2002), Activity of lipases and esterases towards tertiary alcohols: Insights into structure-function relationships, Angew. Chem. Int. Ed., 41, 3211-3213, link. Aktivität von Lipasen und Esterasen gegenüber tertiären Alkoholen: Neue Einblicke in Struktur-Funktions-Beziehungen, Angew. Chem., 114, 3338-3341, link,supporting information: pdf.
[91] Hildebrandt, P., Musidlowska-Persson, A., Bornscheuer, U.T., Altenbuchner, J. (2002), Cloning, functional expression and biochemical characterization of a stereoselective dehydrogenase from Pseudomonas fluorescens DSM50106, Appl. Microbiol. Biotechnol., 59, 483-487.
[90] Bornscheuer, U.T. (2002), Microbial carboxyl esterases - Classification, properties and application in biocatalysis, FEMS Microbiol. Rev. 26, 73-81.
[89] Henke, E., Baumann, M., Musidlowska-Persson, A., Bornscheuer, U.T. (2002), Innovative Methoden zur Erzeugung stereoselektiver Esterasen, BIOforum 4, 218-219.
[88] Yan, Y., Bornscheuer, U.T., Schmid, R.D. (2002), Efficient water removal in lipase-catalyzed esterifications using a low-boiling-point azeotrope, Biotechnol. Bioeng. 78, 31-34, link.
2001
[87] Baumann, M., Stürmer, R., Bornscheuer,
U.T. (2001), A high-throughput-screening method for the identification of
active and enantioselective hydrolases, Angew. Chem. Int. Ed., 40, 4201-4204,
link.
Eine Hochdurchsatz-Screening-Methode zur Identifizierung aktiver und enantioselektiver
Hydrolasen, Angew. Chem. 113, 4329-4333, link.
(HOT PAPER).
[86] Bornscheuer, U.T. (2001), Lipase-catalyzed synthesis of structured triglycerides, Lipid Technology, 7, 105-107.
[85] Yan, Y., Stadler, G., Lutz-Wahl, S., Otto, R.T., Reuss, M., Bornscheuer, U.T., Schmid, R.D. (2001), Regioselective glucose ester synthesis on preparative scale, Eur. J. Lipid Sci. Technol., 103, 583-587, link.
[84] Lange, S., Musidlowska,A., Schmidt-Dannert, C., Schmitt, J., Bornscheuer, U.T. (2001), Cloning,functional expression and characterization of recombinant pig liver esterase, ChemBioChem.,2, 576-582, link.
[83] Musidlowska, A., Lange, S., Bornscheuer, U.T. (2001), Via Overexpression in the yeast Pichia pastoris to enhanced enantioselectivity: New aspects in the application of pig liver esterases, Angew. Chem. Int. Ed., 40, 2851-2853, link. Durch Überexpression in der Hefe Pichia pastoris zu erhöhter Enantioselektivität: Neue Aspekte in der Anwendung von Schweineleberesterase, Angew. Chem., 113, 2934-2936, link.
[82] Hildebrandt, P., Riermeier, T., Altenbuchner, J., Bornscheuer, U.T. (2001), Efficient resolution of prostereogenic arylaliphatic ketones using a recombinant alcohol dehydrogenase from Pseudomonas fluorescens DSM50106 expressed in E. coli, Tetrahedron: Asymmetry, 12, 1207-1210.
[81] Bornscheuer, U.T. (2001), Directed evolution of enzymes for biocatalytic applications, Biocatal. Biotransform., 19, 84-96.
[80] Liu, A. M. F., Somers, N. A., Kazlauskas, R. J., Brush, T. S., Zocher, F., Enzelberger, M. M., Bornscheuer, U. T., Horsman, G. P., Mezzetti, A., Schmidt-Dannert, C., Schmid, R. D. (2001), Mapping the substrate selectivity of new hydrolases using colorimetric screening:lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes, Tetrahedron: Asymmetry, 12, 545-556.
[79] Bornscheuer,U. T.,Pohl, M. (2001), Improved biocatalysts by directed evolution and rationalprotein design, Curr. Opin. Chem. Biol., 5, 137-142 (pdf). With permission from Elsevier Science, single copies of the article may be downloaded and printed for the reader's personal research and study here.
[78] Yan, Y., Bornscheuer, U. T., Stadler, G., Lutz-Wahl, S., Reuss, M., Schmid, R. D. (2001), Production of sugar fatty acid esters by enzymatic esterification in a stirred-tankmembrane reactor: Optimization of parameters by response surface methodology, J. Am. Oil Chem. Soc. 78, 147-152.
2000
[77] Yan, Y., Bornscheuer, U.T., Stadler, G.,
Reuss, R., Schmid, R.D. (2000), Enzymatic productionof biosurfactant 6-O-glucose
fatty acid monoester, J. Deterg. Cosmetics(in Chinese), 23, 71-77.
[76] Baumann, M., Hauer,B.H., Bornscheuer, U.T. (2000), Rapid screening of hydrolases for the enantioselective conversion of "difficult-to-resolve substrates", Tetrahedron: Asymmetry,11, 4781-4790, supporting information (pdf-file).
[75] Bornscheuer, U. T. (2000), Directed evolution of enzymes, Chim. Oggi/ Chem. Today, 18(9), 65-67.
[74] Stander, M. A., Bornscheuer, U. T., Henke, E., Steyn, P. S. (2000), Screening of commercial hydrolases for the degradation of Ochratoxin A, J. Agric. Food Chem., 48, 5736-5739, link.
[73] Peters, H., Schmidt-Dannert, C., Cao, L., Bornscheuer, U.T., Schmid, R.D. (2000), Purification and reconstitution of an integral membrane protein, the photoreaction center of Rhodobacter sphaeroides, using synthetic sugar esters, Biotechniques 28, 1214-1219.
[72] Henke, E., Schuster, S., Yang, H., Bornscheuer, U.T., (2000), Lipase-catalyzed resolution of Ibuprofen, Chem. Month. 131, 633-638.
[71] Otto, R., Scheib, H., Bornscheuer, U.T., Pleiss, J., Syldatk, C., Schmid, R.D. (2000), Substrate specificity of lipase B from Candida antarctica in the synthesis of arlyaliphatic glycolipids, J. Mol. Catal. B: Enzymatic 8,210-211.
1999
[70] Yan, Y., Bornscheuer, U.T., Schmid, R.D.
(1999), Lipase-catalyzed synthesis of vitamin C fattyacid esters, Biotechnol.
Lett.21, 1051-1054.
[69] Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Wohlleben, W., Schmid, R.D. (1999),Epoxide hydrolase activity of Streptomyces strains, J. Biotechnol.77 ,287-292.
[68] Wolfangel, P., Meyer, H.H., Bornscheuer, U.T., Müller, K. (1999), Solid state NMR and FTIR studies on bilayer membranes from 1,2-dioctadec-(14-ynoyl)-sn-glycero-3-phosphatidylcholine, Biochim.Biophys. Acta 1420, 121-138.
[67] Henke, E., Bornscheuer, U.T. (1999), Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay, Biol. Chem. 380, 1029-1033.
[66] Yan, Y., Bornscheuer, U.T., Cao, L., Schmid, R.D. (1999), Lipase-catalyzed solid phase synthesis of sugar fatty acid esters. Removal of by-products by azeotropic distillation, Enzyme Microb. Technol. 25, 725-728.
[65] Bornscheuer, U.T., Altenbuchner, J., Meyer., H.H. (1999), Directed evolution of an esterase: Screening of enzyme libraries based on pH-indicators and a growth assay, Bioorg.Med. Chem.7, 2169-2173.
[64] Schmid, U., Bornscheuer, U.T., Soumanou, M.M., McNeill, G.P., Schmid, R.D. (1999), Highly-selective synthesis of 1,3-oleyl-2-palmitoyl-glycerol by lipase catalysis, Biotechnol. Bioeng. 64, 678-684.
[63] Ji, H.-S., McNiven,S., Yano, K., Ikebukuro, K., Bornscheuer, U.T., Schmid, R.D., Karube, I.(1999), Highly sensitive trilayer piezoelectric odor sensor, Anal. Chim.Acta 387, 39-45.
[62] Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Schmid, R.D. (1999), A colorimetric assay suitable for screening epoxide hydrolase activity, Anal. Chim. Acta 391, 345-351.
[61] Khalameyzer, V., Bornscheuer, U.T. (1999), Overexpression and characterization of an esterase from Streptomyces diastatochromogenes, Biotechnol. Lett.21, 101-104.
[60] Yang, H., Henke, E., Bornscheuer, U.T. (1999), Highly efficient double enantioselection by lipase-catalyzed transesterification of (R,S)-carboxylic acid vinyl esters with ( R,S)-1-phenylethanol,Tetrahedron:Asymmetry10, 957-960.
[59] Yang, H., Henke, E., Bornscheuer, U.T. (1999), The use of vinyl esters significantly enhancedenantioselectivities and reaction rates in lipase-catalyzed resolutions of arlyaliphatic carboxylic acids, J. Org. Chem. 64, 1709-1712.
[58] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1999), Lipase-catalyzed solid-phase synthesis of sugar esters. Influence of immobilization on productivity and stability of the enzyme, J. Mol. Catal. B: Enzymatic 6, 279-285.
[57] Khalameyzer, V., Fischer, I., Bornscheuer, U.T., Altenbuchner, J. (1999), Screening, nucleotide sequence and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity toward lactones, Appl. Environm. Microbiol. 65,477-482.
[56] Minning, S., Weiss,A., Bornscheuer, U.T., Schmid, R.D. (1999), Determination of peracid and putative enzymatic peracid formation by an easy colorimetric assay, Anal.Chim.Acta 378, 295-300.
[55] Soumanou, M.M., Bornscheuer, U.T., Schmid, U., Schmid, R.D. (1999), Crucial role of support and water activity on the lipase-catalyzed synthesis of structured triglycerides, Biocatal. Biotransform. 16, 443-459.
1998
[54] Cao, L., Bornscheuer, U.T., Schmid, R.D.
(1998), Lipase-catalyzed solid phase synthesis of sugar esters, IV: Selectivity
of lipases towards primary and secondary hydroxyl groups in carbohydrates,
Biocatal. Biotransform. 16, 249-257.
[53] Bornscheuer, U.T. (1998), Directed evolution of enzymes, Angew. Chem. / Angew. Chem. Int. Ed. Engl. 110 / 37, 3285-3288 / 3105-3108.
[52] Schmid, U., Bornscheuer, U.T., Soumanou, M.M., McNeill, G.P., Schmid, R.D. (1998), Optimization of the reaction conditions in the lipase-catalyzed synthesis of structured triglycerides, J. Am. Oil Chem. Soc. 75, 1527-1531.
[51] Otto, R.T., Bornscheuer, U.T., Scheib, H., Pleiss, J., Syldatk, C., Schmid, R.D. (1998), Lipasecatalyzed esterification of unusual substrates: Synthesis of glucuronic acid and ascorbic acid (vitamin C) esters, Biotechnol. Lett. 20,1091-1094.
[50] Takahashi, S., Ueda, M., Atomi, H., Beer, H.D., Bornscheuer, U.T., Schmid, R.D., Tanaka, A. (1998), Extracellular production of active Rhizopus oryzae lipase by Saccharomyces cerevisiae, J. Ferment. Bioeng. 86, 164-168.
[49] Otto, R., Bornscheuer, U.T., Syldatk, C., Schmid, R.D. (1998), Lipase-catalyzed synthesis of arylaliphatic esters of b-D(+)-glucose, alkyl- and arylglucosides and characterizationof their surfactant properties, J. Biotechnol. 64, 231-237.
[48] Beer, H.D., Bornscheuer, U.T., McCarthy, J.E.G., Schmid, R.D. (1998), Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae, Biochim.Biophys. Acta 1399, 173-180.
[47] Bornscheuer, U., Heinz, E. (1998), Toward new fats, oils through biotechnology, Inform 9, 485-488.
[46] Wagegg, T., Enzelberger, M.M., Bornscheuer, U.T., Schmid, R.D. (1998), The use of methoxy acetoxyesters significantly enhances reaction rates in the lipase-catalyzed preparation of optical pure 1-(4-chloro-phenyl) ethyl amines, J. Biotechnol. 61,75-78.
[45] Enzelberger, M.M., Zocher, F., Schmidt-Dannert, C., Bornscheuer, U.T., Schmid, R.D., Hauer, B., Eipel, H. (1998), Singularization of enzyme mutants by use of a cell sorter andgreen fluorescent protein, BIOforum 4, 192-194.
[44] Otto, R.T., Bornscheuer, U.T., Syldatk, C., Schmid, R.D. (1998), Synthesis of aromatic n-alkyl-glucoside esters in a coupled ß-glucosidase and lipase reaction, Biotechnol. Lett. 20, 437-440.
[43] Soumanou, M.M., Bornscheuer, U.T., Schmid, R.D. (1998), Two-step enzymatic reaction for the synthesisof pure structured triglycerides, J. Am. Oil Chem. Soc. 75,703-710.
[42] Soumanou, M.M., Bornscheuer, U.T., Schmid, U., Schmid, R.D. (1998), Synthesis of structured triglycerides by lipase catalysis, Fett/Lipid 100, 156-160.
[41] Krebsfänger, N., Zocher, F., Altenbuchner, J., Bornscheuer, U.T. (1998), Characterization, and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens, Enzyme Microb. Technol. 21, 641-646.
[40] Yano, K., Bornscheuer, U.T., Schmid, R.D., Yoshitake, H., Ikebukuro, K., Yokoyama, K., Masuda,Y., Karube, I. (1998), Development of odorant sensor using polymer-coatedquartz crystals immobilized with unusual lipids, Biosensors Bioelectronics 13,397-405.
[39] Zocher, F., Krebsfänger, N., Yoo, O.J., Bornscheuer, U.T. (1998), Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens, J. Mol. Catal. B, Enzymatic 5, 187-190.
[38] Bornscheuer, U.T., Altenbuchner, J., Meyer, H.H. (1998), Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of Epothilones, Biotechnol. Bioeng. 58, 554-559, link.
[37] Krebsfänger, N., Schierholz, K., Bornscheuer, U.T. (1998), Enantioselectivity of a recombinant esterase from Pseudomonas fluorescens towards alcohols and carboxylic acids, J. Biotechnol. 60, 105-111.
[36] Bornscheuer, U.T., Enzelberger, M.M., Altenbuchner, J., Meyer, H.H. (1998), Mutator strain Epicuriancoli XL1-red generates esterase variants, Strategies 11, 16-17.
[35] Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D., Schwarz, D. (1998), Branched phosphatidylcholines stimulate activity of cytochrome P450SCC (CYP11A1)in phospholipid vesicles by enhancing cholesterol binding, membrane incorporation, and protein exchange, J. Biol. Chem. 273, 1380-1286.
1997
[34] Yano, K., Yoshitake, H., Bornscheuer,
U.T., Schmid, R.D., Ikebukuro, K., Yokoyama, K., Masuda, Y., Karube, I. (1997),
Development of a chemical vapor sensor using piezoelectric quartz crystals
coated with unusual lipids, Anal. Chim. Acta 340 ,41-48.
[33] Thude, S., Shukun, L., Said, M.B., Bornscheuer, U.T. (1997), Lipase-catalyzed synthesis of monoacylglycerides by glycerolysis of campher tree seed oil and cocoa-butter, Fett/Lipid 99,246-250.
[32] Blüher, A., Grube, A., Bornscheuer, U., Banik, G. (1997), A reappraisal of the enzyme lipase for removing drying-oil stains on paper, Paper Conservator 21 ,37-47.
[31] Soumanou, M.M., Bornscheuer, U.T., Menge, U., Schmid, R.D. (1997), Synthesis of structured triglycerides from peanut oil with immobilized lipase, J. Am. Oil Chem. Soc. 74,427-433.
[30] Cao, L., Fischer, A., Bornscheuer, U.T., Schmid, R.D. (1997), Lipase-catalyzed solid phase preparation of sugar fatty acid esters, Biocatal. Biotransform. 14, 269-283.
[29] Schwarz, D., Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Possible involvement of nonbilayer lipids in the stimulation of the activity ofcytochrome P450SCC (CYP11A1) and its propensity to induce vesicle aggregation,Chem. Phys. Lipids 85, 91-99.
[28] Pisch, S., Bornscheuer, U., Meyer, H.H., Schmid, R.D. (1997), Properties of unusual phospholipids IV: Chemoenzymatic synthesis of phospholipids bearing acetylenic fatty acids, Tetrahedron 53, 14627-14634.
[27] Negelmann, L., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Properties of unusual phospholipids III: Synthesis, monolayer investigations and DSC studies of hydroxy octadeca(e)noic acids and diacylglycerophosphocholines derived therefrom, Chem. Phys. Lipids 90, 117-134.
[26] Schwarz, D., Kisselev, P., Pfeil, W., Pisch, S., Bornscheuer, U., Schmid, R.D. (1997), Evidencethat nonbilayer phase propensity of the membrane is important for the sidechain cleavage activity of cytochrome P450SCC (CYP11A1), Biochemistry 36,14262-14270.
[25] Enzelberger, M.M., Bornscheuer, U.T., Gatfield, I., Schmid, R.D. (1997), Lipase-catalysed resolution of g-and d -lactones, J. Biotechnol. 56, 129-133.
1996
[24] Gaziola, L., Bornscheuer, U., Schmid,
R.D. (1996), A rapid and effective separation of enantiomers of glycerol derivatives
by gas chromatography and their lipase-catalyzed biotransformation, Enantiomer1,
49-54.
[23] Wünsche, K., Schwaneberg, U., Bornscheuer, U.T., Meyer, H.H. (1996), Chemoenzymic route to b-blockers via 3-hydroxy esters, Tetrahedron: Asymmetry 7, 2017-2022.
[22] Bornscheuer, U., Gaziola, L., Schmid, R.D. (1996), Application of vinyl esters for the lipase-catalyzed high-yield synthesis of monoacylglycerols, Ann. N.Y. Acad. Sci. 799,757-761.
[21] Lampe, T.F.J., Hoffmann, H.M.R., Bornscheuer, U.T. (1996), Lipase mediated desymmetrization of meso 2,6- di(acetoxymethyl)tetrahydropyran-4-one derivatives. An innovative route to enantiopure 2,4,6-trifunctionalized C-glycosides, Tetrahedron:Asymmetry 7, 2889-2900.
[20] Bornscheuer, U., Capewell, A., Wendel, V., Scheper, T. (1996), Online determination of the conversion in a lipase-catalyzed kinetic resolution in supercritical carbon dioxide, J. Biotechnol. 46, 139-143.
[19] Heidt, M., Bornscheuer, U., Schmid, R.D. (1996), Studies on the enantioselectivity in the lipase-catalyzed synthesis of monoacylglycerols from the isopropylidene glycerol, Biotechnol. Tech. 10, 25-30.
[18] Cao, L., Bornscheuer, U.T., Schmid, R.D. (1996), Lipase-catalyzed solid-phase synthesis of sugar esters, Fett/Lipid 98, 332-335.
[17] Capewell, A., Wendel, V., Bornscheuer, U., Meyer, H.H., Scheper, T. (1996), Lipase-catalyzed kinetic resolution of 3-hydroxy esters in organic solvents and supercritical carbon dioxide, Enzyme Microb. Technol. 19, 181-186.
1995
[16] Bornscheuer, U., Herar, A., Capewell,
A., Wendel, V., Kreye, L., Scheper, T., Voß, E., Wünsche, K., Meyer,
H.H. (1995), Lipase-catalyzed kinetic resolution of 3-hydroxyesters: Optimization,
batch, and continuous reactions, Ann. N.Y. Acad.Sci.750, 215-221.
[15] Bornscheuer, U.T., Yamane, T. (1995), Fatty acid vinyl esters as acylating agents: a new method for the enzymic synthesis of monoacylglycerols, J. Am. Oil Chem. Soc.72 ,193-197.
[14] Bornscheuer, U.T. (1995), Lipase-catalyzed syntheses of monoacylglycerols, Enzyme Microb. Technol. 17,578-586.
[13] Bornscheuer, U. (1995), Lipase-catalyzed synthesis of monoglycerides, Fat Sci. Technol. 97,241-249.
[12] Ballesteros, A., Bornscheuer, U., Capewell, A., Combes, D., Condoret, J.-S., König, K., Kolisis, F.N., Marty, A., Menge, U., Scheper, T., Stamatis, H., Xenakis, A. (1995), Review article: Enzymes in non-conventional phases, Biocatal. Biotransform. 13, 1-42.
[11] Heß, R., Bornscheuer, U., Capewell, A., Scheper, T. (1995), Lipase-catalyzed synthesis of monostearoylglycerol in organic solvents from 1,2-O-isopropylidene glycerol, Enzyme Microb. Technol. 17, 725-728.
1994
[10] Bornscheuer, U., Stamatis, H., Xenakis,
A., Yamane, T., Kolisis, F.N. (1994), A comparison of different strategies
for lipase-catalyzed synthesis of partial glycerides, Biotechnol. Lett. 16,
697-702.
[9] Bornscheuer, U.T., Yamane, T. (1994), Activity and stability of lipase in the solid-phase glycerolysis of triolein, Enzyme Microb. Technol. 16, 864-869.
[8] Bornscheuer, U., Reif, O.-W., Lausch, R., Freitag, R., Scheper, T., Kolisis, F.N., Menge, U. (1994), Lipase of Pseudomonas cepacia for biotechnological purposes: purification, crystallization and characterization, Biochim. Biophys. Acta 1201, 55-60.
[7] Kreye, L., Herar, A., Bornscheuer, U., Scheper, T. (1994), Enzymic conversions in organic solvents in batch and continuous systems, Fat Sci. Technol. 96, 246-251.
1993
[6] Stamatis, H., Xenakis, A., Bornscheuer,
U., Scheper, T., Menge, U., Kolisis, F.N. (1993), Pseudomonas cepacia lipase:
esterification reactionsin AOT microemulsion systems, Biotechnol. Lett. 15,
703-708.
[5] Bornscheuer, U., Herar, A., Kreye, L., Wendel, V., Capewell, A., Meyer, H.H., Scheper, T., Kolisis, F.N. (1993), Factors affecting the lipase catalyzed transesterification reactions of 3-hydroxy esters in organic solvents, Tetrahedron:Asymmetry 4, 1007-1016.
1992
[4] Capewell, A., Bornscheuer, U., Herar, A.,
Scheper, T., Meyer, H.H., Kolisis, F. (1992), A comparison of enzymic reactions
in aqueous, organic and supercritical phases, DECHEMA Biotechnol. Conf. 5,
57-60.
[3] Bornscheuer, U., Schapöhler, S., Scheper, T., Schügerl, K., König, W.A. (1992), Application of enantioselective capillary gas chromatography in lipase-catalyzed transesterification reactions in organic media, J. Chromatogr. 606, 288-290.
[2] Bornscheuer, U., Capewell, A., Scheper, T., Meyer, H.H. (1992), A comparison of enzymic reactions in aqueous, organic, and supercritical phases, Ann. N.Y. Acad. Sci. 672, 336-342.
1991
[1] Bornscheuer, U., Schapöhler, S., Scheper, T., Schügerl, K. (1991),
Influences of reaction conditions on the enantioselective transesterification
using Pseudomonas cepacia lipase, Tetrahedron: Asymmetry 2, 1011-1014.
News
GRC Biocatalysis:
The program of the GRC Biocatalysis 2010 conference is online.
Research in Japan:
Prof. Bornscheuer is currently in Japan as JSPS fellow.
25th Ph.D student finished:
Dr. Matthias Höhne is the 25th Ph.D. student from the Bornscheuer group.
Enzymicals AG launched:
The company Enzymicals AG - a spin-off from the Bornscheuer group - has started its business.
Author profile:
An author profile about Uwe Bornscheuer has been published in Angew. Chem. Int. Ed.
ChemCatChem:
Prof. Bornscheuer is Chairmen of the Editorial Board of the new journal ChemCatChem launched by Wiley-VCH
VentureCup-MV:
A team of our group was the winner at the VentureCup-MV 2009.
Flyer available:
English version (pdf)
German version (pdf)
Recently published:
Edited by S. Lutz and U.T. Bornscheuer, published by Wiley-VCH.
Cluster Partner:
Partner in the 'White Biotechnology' cluster 'Biokatalyse2021' funded by the BMBF.
Contact
Prof. Dr.
Uwe Bornscheuer
Institute of Biochemistry
Dept. of Biotechnology & Enzyme Catalysis
Felix-Hausdorff-Str. 4
D-17487 Greifswald/ Germany
Phone:
+49 (0)3834-86-4367
+49 (0)3834-86-4391 (Secr.)
Fax:
+49 (0)3834-86-80066
E-Mail:
uwe.bornscheuer
